A comparison of complete mitochondrial DNA sequences of Selys, 1869 (Odonata: Calopterygidae) from three different populations (one allopatric and two sympatric).

In Japan, two closely-related damselflies, Selys, 1869 (Odonata: Calopterygidae) and Selys-Longchamps (Odonata: Calopterygidae), 1853, coexist, and they exhibit geographic variations in wing color, body size, and habitat preference. In this study, we analyzed the complete mitochondrial genome of from Saga Prefecture, Japan (sympatric populations that exhibit wing color polymorphism), and compared the genome with that exhibit monomorphic orange wing color. The mitochondrial genome of from Saga Prefecture was identified as a circular molecule of 15,488 bp, similar to that found in other populations. It was predicted to contain 13 protein-coding (PCG), 22 tRNA, and two rRNA genes, along with one A + T-rich control region. Among the PCGs, and , and , and , and and shared seven, one, seven, and one nucleotides, respectively. The initiation codon ATG was found in eight genes, ATC in four, and ATT in one, while the termination codons TAA, TAG, TA, and T were observed in seven, one, two, and three genes, respectively. All the tRNA genes possessed a cloverleaf secondary structure, except for that lacks the TΨC loop. The average AT content of mitochondrial genome was 66.06%. From a phylogenetic analysis, the loss of wing color polymorphism in monomorphic sympatric populations is likely to occur with the coexistence of two species.