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利用新一代测序技术测定的岩蟹(林奈,1758年)完整线粒体基因组。

The complete mitochondrial genome of rubble crab (Linnaeus, 1758) using next-generation sequencing.

作者信息

Yang Mingqiu, He Yugui, Liu Hongtao

机构信息

Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou, China.

出版信息

Mitochondrial DNA B Resour. 2019 Oct 3;4(2):3320-3321. doi: 10.1080/23802359.2019.1673255.

Abstract

In this study, the complete mitochondrial genome of the rubble carb was determined using Illumina next-generation sequencing. The circular mitogenome is 15,737 bp, with 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes and one control region. The base composition is significantly biased (A, G, T, and C was 35.5%, 10.2%, 34.9%, and 19.4%, respectively) with A + T contents of 70.4%. Five microsatellites were identified in the mitogenome located in ND1, ND4, 16S genes and D-loop region. Phylogenetic tree showed that is first clustered with and , which revealed that Parthenopoidea has a close affinity with Eriphioidea in Heterotremata.

摘要

在本研究中,使用Illumina下一代测序技术测定了碎石蟹的完整线粒体基因组。环状线粒体基因组为15737 bp,包含13个蛋白质编码基因(PCGs)、22个tRNA基因、2个rRNA基因和1个控制区。碱基组成存在显著偏差(A、G、T和C分别为35.5%、10.2%、34.9%和19.4%),A+T含量为70.4%。在线粒体基因组中鉴定出5个微卫星,分别位于ND1、ND4、16S基因和D-loop区域。系统发育树显示,[此处原文缺失具体物种名]首先与[此处原文缺失具体物种名]和[此处原文缺失具体物种名]聚类,这表明在异孔亚目中,短尾派与绵蟹派具有密切的亲缘关系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d4f/7707263/aec6870dba8e/TMDN_A_1673255_F0001_B.jpg

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