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独行菜(L.)的完整叶绿体基因组序列及其在十字花科中的系统发育分析。

The complete chloroplast genome sequence of garden cress ( L.) and its phylogenetic analysis in Brassicaceae family.

作者信息

Zhu Bin, Gao Zuomin, Luo Xi, Feng Qun, Du Xuye, Weng Qingbei, Cai Mengxian

机构信息

School of Life Sciences, Guizhou Normal University, Guiyang, People's Republic of China.

出版信息

Mitochondrial DNA B Resour. 2019 Oct 16;4(2):3601-3602. doi: 10.1080/23802359.2019.1677527.

Abstract

Garden cress, L., is not only an important vegetable which is cultivated in the entire world, but also a widely used folk medicine for the treatment of hyperactive airways disorders. However, as a member of Brassicaceae, few studies have been carried out on its phylogenetic relationship with other Brassicaceae members. Herein, the complete chloroplast (cp) genome of garden cress wa deciphered by the combination of Illumina Hiseq and PacBio Hiseq Platform after extracting of its cp DNA. The cp genome showed a typically quadripartite cycle with 154997 bp, including a pair of inverted repeats (IRa and IRb) of 26491 bp intersected by a large single copy (LSC) region of 84007 bp and a small single copy (SSC) region of 18008 bp. Totally, 128 unique genes were assembled in this cp genome, including 83 protein genes, 37 tRNAs and 8 rRNAs. A total of 73 simple sequence repeats (SSRs) with a length of at least 10 bp were detected. Phylogenetic analysis based on 30 cp genome of Brassicaceae family showed that the was closely related to . This study provides important information for future evolution, genetic and molecular biology studies of .

摘要

水田芥(L.)不仅是一种在全球范围内广泛种植的重要蔬菜,也是一种广泛用于治疗气道功能亢进疾病的民间药物。然而,作为十字花科的一员,关于其与其他十字花科成员系统发育关系的研究却很少。在此,通过提取其叶绿体DNA,结合Illumina Hiseq和PacBio Hiseq平台,解析了水田芥完整的叶绿体(cp)基因组。该cp基因组呈现典型的四分体环状结构,大小为154997 bp,包括一对26491 bp的反向重复序列(IRa和IRb),被一个84007 bp的大单拷贝(LSC)区域和一个18008 bp的小单拷贝(SSC)区域隔开。该cp基因组总共组装了128个独特基因,包括83个蛋白质基因、37个tRNA和8个rRNA。共检测到73个长度至少为10 bp的简单序列重复(SSR)。基于30个十字花科cp基因组的系统发育分析表明,该物种与[具体物种名称缺失]密切相关。本研究为[具体物种名称缺失]未来的进化、遗传和分子生物学研究提供了重要信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8840/7710307/4fee20ede4cb/TMDN_A_1677527_F0001_B.jpg

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