Zhu Bin, Luo Xi, Gao Zuomin, Feng Qun, Hu Lijuan, Weng Qingbei
School of Life Sciences, Guizhou Normal University, Guiyang, People's Republic of China.
Mitochondrial DNA B Resour. 2019 Nov 18;4(2):4085-4086. doi: 10.1080/23802359.2019.1691947.
Rchb.f., a native species to China, is widely used as an important garden flower and a traditional Chinese medicine. Herein, the complete chloroplast (cp) genome of was deciphered by high-throughput sequencing. The cp genome exhibited a typical quadripartite cycle of 151,686 bp in length, comprising of a pair of inverted repeats (IRa and IRb) of 26,293 bp which were intersected by a large single copy (LSC) region of 84,749 bp and a small single copy (SSC) region of 14,351 bp. A total of 126 genes were assembled in this cp genome, including 78 protein genes, 40 tRNA genes and 8 rRNA genes. Among these genes, 86 genes (22 tRNAs and 64 coding genes) were single copy, the rest were two-copy genes, and the average of GC content of the whole genome is 37.55%. Phylogenetic trees showed that the was closely related to . This study provides molecular information for future evolution, genetic and molecular biology studies of .
Rchb.f.是中国本土物种,被广泛用作重要的园林花卉和传统中药。在此,通过高通量测序解析了其完整的叶绿体(cp)基因组。该cp基因组呈现出典型的四分体结构,长度为151,686 bp,由一对26,293 bp的反向重复序列(IRa和IRb)组成,它们被一个84,749 bp的大单拷贝(LSC)区域和一个14,351 bp的小单拷贝(SSC)区域隔开。该cp基因组共组装了126个基因,包括78个蛋白质基因、40个tRNA基因和8个rRNA基因。在这些基因中,86个基因(22个tRNA和64个编码基因)为单拷贝,其余为双拷贝基因,全基因组的GC含量平均为37.55%。系统发育树表明,该物种与……密切相关。本研究为该物种未来的进化、遗传和分子生物学研究提供了分子信息。 (注:原文中部分物种名缺失,翻译时保留了原文格式)