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(兰科)的完整叶绿体基因组序列及其系统发育分析。

The complete chloroplast genome sequence of (Orchidaceae) and its phylogenetic analysis.

作者信息

Zhu Bin, Luo Xi, Gao Zuomin, Feng Qun, Hu Lijuan, Weng Qingbei

机构信息

School of Life Sciences, Guizhou Normal University, Guiyang, People's Republic of China.

出版信息

Mitochondrial DNA B Resour. 2019 Nov 18;4(2):4085-4086. doi: 10.1080/23802359.2019.1691947.

Abstract

Rchb.f., a native species to China, is widely used as an important garden flower and a traditional Chinese medicine. Herein, the complete chloroplast (cp) genome of was deciphered by high-throughput sequencing. The cp genome exhibited a typical quadripartite cycle of 151,686 bp in length, comprising of a pair of inverted repeats (IRa and IRb) of 26,293 bp which were intersected by a large single copy (LSC) region of 84,749 bp and a small single copy (SSC) region of 14,351 bp. A total of 126 genes were assembled in this cp genome, including 78 protein genes, 40 tRNA genes and 8 rRNA genes. Among these genes, 86 genes (22 tRNAs and 64 coding genes) were single copy, the rest were two-copy genes, and the average of GC content of the whole genome is 37.55%. Phylogenetic trees showed that the was closely related to . This study provides molecular information for future evolution, genetic and molecular biology studies of .

摘要

Rchb.f.是中国本土物种,被广泛用作重要的园林花卉和传统中药。在此,通过高通量测序解析了其完整的叶绿体(cp)基因组。该cp基因组呈现出典型的四分体结构,长度为151,686 bp,由一对26,293 bp的反向重复序列(IRa和IRb)组成,它们被一个84,749 bp的大单拷贝(LSC)区域和一个14,351 bp的小单拷贝(SSC)区域隔开。该cp基因组共组装了126个基因,包括78个蛋白质基因、40个tRNA基因和8个rRNA基因。在这些基因中,86个基因(22个tRNA和64个编码基因)为单拷贝,其余为双拷贝基因,全基因组的GC含量平均为37.55%。系统发育树表明,该物种与……密切相关。本研究为该物种未来的进化、遗传和分子生物学研究提供了分子信息。 (注:原文中部分物种名缺失,翻译时保留了原文格式)

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f90c/7707769/60c693a805f4/TMDN_A_1691947_F0001_B.jpg

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