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建兰(兰科)的完整叶绿体基因组及其系统发育意义。

The complete chloroplast genome of S. S. Chien (Orchidaceae) and its phylogenetic implication.

作者信息

Yi Xiangui, Li Mingzhi, Chen Lin, Wang Xianrong

机构信息

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing Shi, China.

College of Biology and Environment, Nanjing Forestry University, Nanjing Shi, China.

出版信息

Mitochondrial DNA B Resour. 2019 Dec 12;5(1):238-239. doi: 10.1080/23802359.2019.1699464.

Abstract

S. S. Chien is mainly distributed in the mid-subtropical hilly areas of central and eastern China at an altitude of 200-1700 m. It has important academic significance in the orchid phylogeny, and is an excellent wild flower and precious medicinal plant. The species was once abundant but has become rare and endangered in recent years and has been identified as Endangered (EN) under criteria A2c (The IUCN Red List and Threatened Specis) because of the habitat fragmentation and unduly commercial collections. In this study, the complete chloroplast (cp) genome sequence of was determined using next-generation sequencing. The entire cp genome was determined to be 156,818 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 84,847 and 18,141bp, respectively, which were separated by a pair of 26,915 bp inverted repeat (IR) regions. The genome contained 133 genes, including 87 protein-coding genes, 38 tRNA genes, and eight rRNA genes.The overall GC content of the genome is 37.1%. A phylogenetic tree reconstructed by 48 chloroplast single-copy coding gene reveals that is closely related with .

摘要

建兰主要分布于中国中部和东部海拔200 - 1700米的中亚热带丘陵地区。它在兰花系统发育中具有重要的学术意义,是优良的野生花卉和珍贵的药用植物。该物种曾经数量众多,但近年来变得稀有且濒危,由于栖息地破碎化和过度商业采集,根据标准A2c(《世界自然保护联盟红色名录和受威胁物种》)被认定为濒危(EN)。在本研究中,利用二代测序技术测定了建兰的完整叶绿体(cp)基因组序列。整个cp基因组长度为156,818 bp。它包含分别为84,847 bp和18,141 bp的大单拷贝(LSC)和小单拷贝(SSC)区域,这两个区域被一对26,915 bp的反向重复(IR)区域隔开。该基因组包含133个基因,包括87个蛋白质编码基因、38个tRNA基因和8个rRNA基因。基因组的总体GC含量为37.1%。由48个叶绿体单拷贝编码基因重建的系统发育树表明,建兰与[未提及的物种]密切相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9daf/7748693/a01d2446752d/TMDN_A_1699464_F0001_B.jpg

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