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R. Br.()的完整叶绿体基因组及其系统发育意义。

The complete chloroplast genome of R. Br. () and its phylogenetic implication.

作者信息

Wang Fei-Fei, Huang Dao-Jun, Qin Bao-Li, Wang Xia-Wen, Jin Qian, Zhang Zhi-Hui, Wang Xin-Hai

机构信息

Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian, China.

出版信息

Mitochondrial DNA B Resour. 2020 Jan 22;5(1):766-767. doi: 10.1080/23802359.2020.1715865.

DOI:10.1080/23802359.2020.1715865
PMID:33366741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7748759/
Abstract

The genus () is an annual parasitic twining herb. There are about 200 species in this genus, which are widely distributed in tropical and subtropical areas. is mainly parasitic on crops bringing significant losses to the production of agriculture. Furthermore, dried seeds of and are used as a Chinese traditional herbal medicine. Despite the importance of species, it is difficult to distinguish these plants by the naked eye. Moreover, plastid sequence information available for species is limited. In this study, the complete chloroplast (cp) genome sequence of was determined using next-generation sequencing. The entire cp genome was determined to be 85,263 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 50,384 and 6727 bp, respectively, which were separated by a pair of 14,076 bp inverted repeat (IR) regions. The genome contained 98 genes, including 61 protein-coding genes, 29 tRNA genes, and 4 rRNA genes. The overall GC content of the genome is 37.8%. A phylogenetic tree reconstructed by 26 chloroplast genomes reveals that is most related with in , with bootstrap support values of 100%.

摘要

(该属)是一种一年生寄生缠绕草本植物。该属约有200种,广泛分布于热带和亚热带地区。(该属植物)主要寄生于农作物上,给农业生产带来重大损失。此外,(该属植物)和(另一属植物)的干燥种子被用作中国传统草药。尽管该属植物很重要,但仅凭肉眼很难区分这些植物。此外,可用于该属植物的质体序列信息有限。在本研究中,利用二代测序技术测定了(某植物)的完整叶绿体(cp)基因组序列。整个cp基因组长度测定为85,263 bp。它包含分别为50,384 bp和6,727 bp的大单拷贝(LSC)和小单拷贝(SSC)区域,这两个区域被一对14,076 bp的反向重复(IR)区域隔开。该基因组包含98个基因,包括61个蛋白质编码基因、29个tRNA基因和4个rRNA基因。基因组的总体GC含量为37.8%。由26个叶绿体基因组重建的系统发育树表明,(某植物)与(另一植物)在(某属)中关系最为密切,自展支持值为100%。

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