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(兰科)一种濒危药用和观赏植物的完整质体基因组序列。

The complete plastome sequence of (Orchidaceae): an endangered medicinal and ornamental plant.

作者信息

Zhang Xiao, Li Huaizhu, Huang Huan, Wang Qiaoli

机构信息

School of Chemistry and Chemical Engineering, Xianyang Normal University, Xianyang City, PR China.

出版信息

Mitochondrial DNA B Resour. 2020 Jan 10;5(1):468-469. doi: 10.1080/23802359.2019.1704643.

Abstract

(Thunb. ex A. Marray) Lindl belongs to the genus within family Orchidaceae, is an endangered herbal species in the East Asia area. In this study, the complete plastome sequence (cpDNA) of was determined by next-generation Illumina sequencing. The cpDNA of this herbal plant is 155,409 bp in size, with a pair of inverted repeat (IR) regions of 26,933 bp that separate a large single-copy (LSC) region of 84,049 bp and a small single-copy (SSC) region of 17,494 bp. The GC content in plastome is 36.9%, and the IR region (43.2%) is higher than that of the LSC and SSC region (34.4% and 29.7%, respectively), which is similar with other Orchidaceae plastomes. The assembled plastome encoded 133 genes, which included 38 tRNA genes, 8 rRNA genes, and 87 protein-coding genes. A total of 24 species were used to construct the phylogenetic relationships among and other related species within Orchidaceae. The results showed that is closely related to .

摘要

(拇指兰属,以A. 马里命名)林德尔属于兰科下的一个属,是东亚地区的一种濒危草本植物。在本研究中,通过下一代Illumina测序确定了该植物的完整叶绿体基因组序列(cpDNA)。这种草本植物的cpDNA大小为155,409 bp,有一对26,933 bp的反向重复(IR)区域,将一个84,049 bp的大单拷贝(LSC)区域和一个17,494 bp的小单拷贝(SSC)区域分开。叶绿体基因组中的GC含量为36.9%,IR区域(43.2%)高于LSC和SSC区域(分别为34.4%和29.7%),这与其他兰科叶绿体基因组相似。组装的叶绿体基因组编码133个基因,包括38个tRNA基因、8个rRNA基因和87个蛋白质编码基因。总共使用了24个物种来构建该植物与兰科其他相关物种之间的系统发育关系。结果表明,该植物与某植物密切相关。 (原文中部分植物名称未给出具体中文,保留英文)

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0338/7748498/5a7f8b2796b1/TMDN_A_1704643_F0001_B.jpg

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