Kim Young-Kee, Kwak Myoung Hai, Chung Myong Gi, Kim Hoe-Won, Jo Sangjin, Sohn Jung-Yeon, Cheon Se-Hwan, Kim Ki-Joong
Division of Life Sciences, Korea University, Seoul, Korea.
Department of Plant Resources, National Institute of Biological Resources, Incheon, Korea.
Mitochondrial DNA B Resour. 2017 Oct 17;2(2):711-713. doi: 10.1080/23802359.2017.1390409.
In this study, we report the complete chloroplast sequence of (Maxim.) Makino (Orchidaceae) (NCBI acc. no. KX871235), which is an endangered plant species currently protected by the National Law of Korea. The gene order and content of the plastome are similar to those of a typical orchid plastome. The 11 genes are pseudogenized or lost completely from the plastome of The plastome contains 102 genes, of which 68 are protein-coding genes, 30 and four are transfer RNA (tRNA) and ribosomal RNA (rRNA) genes, respectively. Sixteen genes contain one intron and two genes (P and 3) have two introns. The complete plastome is 142,996 bp long and consists of one large and small single copy each of 81,669 and 10,969 bp, respectively, separated by two inverted repeats of 25,179 bp. The AT content of the plastome is 62.6%. Sixty-five simple sequence repeat (SSR) loci, consisting of 56 mono-SSR, seven di-SSR, and two tri-SSR are scattered along the plastome. Some of these plastome SSR loci may be useful for developing genetic markers for the populations. Phylogenetic analysis has shown that is a member of the Malaxideae. The genus forms a monophyletic clade with .
在本研究中，我们报道了（马克西姆）牧野（兰科）的完整叶绿体序列（NCBI登录号：KX871235），它是一种目前受韩国国家法律保护的濒危植物物种。该植物叶绿体基因组的基因顺序和内容与典型的兰花叶绿体基因组相似。11个基因在该植物的叶绿体基因组中已假基因化或完全丢失。该叶绿体基因组包含102个基因，其中68个是蛋白质编码基因，30个和4个分别是转运RNA（tRNA）和核糖体RNA（rRNA）基因。16个基因含有一个内含子，两个基因（P和3）有两个内含子。完整的叶绿体基因组长度为142,996 bp，由一个81,669 bp的大单拷贝和一个10,969 bp的小单拷贝组成，中间由两个25,179 bp的反向重复序列隔开。该植物叶绿体基因组的AT含量为62.6%。65个简单序列重复（SSR）位点，包括56个单SSR、7个双SSR和2个三SSR，沿该植物叶绿体基因组分散分布。这些叶绿体基因组SSR位点中的一些可能有助于开发该植物种群的遗传标记。系统发育分析表明，该植物是沼兰亚族的成员。该属与形成一个单系类群。
