（鞘翅目：叶甲科：叶甲亚科）线粒体基因组及系统发育分析
Mitochondrial genome of (Coleoptera: Chrysomelidae: Eumolpinae) and phylogenetic analysis.
作者信息
Feng Run-Qiu, Zhang Li-Jun, Li Min, Liu Jia, Wen Chun-Li, Yuan Ming-Long
机构信息
Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou, People's Republic of China.
出版信息
Mitochondrial DNA B Resour. 2020 Jan 16;5(1):667-668. doi: 10.1080/23802359.2020.1711823.
Here, we determined the nearly complete mitochondrial genome (mitogenome) of (Coleoptera: Chrysomelidae: Eumolpinae), an important insect pest on in Northwestern China. This mitogenome was 14,451 bp long, encoding 13 protein-coding genes (PCGs), 21 transfer RNA genes (tRNAs), and 2 ribosomal RNA genes. The mitogenome presented an A + T content of 75.11%, with a positive AT-skew (0.064) and a negative GC-skew (-0.192). Ten PCGs started with a typical ATN codon, whereas the remaining three PCGs started with AAC () and TTG ( and ). All tRNAs had a typical secondary cloverleaf structure, except for which lacked the dihydrouridine arm. Bayesian phylogenetic analysis based on the nucleotide sequences of 13 PCGs recovered a phylogeny within Chrysomelidae: (((Chrysomelinae + Galerucinae), (((Eumolpinae, Lamprosomatinae), Cassidinae), Criocerinae)), Bruchinae).
在此，我们测定了中国西北地区一种重要害虫——（鞘翅目：叶甲科：叶甲亚科）的近乎完整的线粒体基因组（线粒体基因组）。该线粒体基因组长度为14,451 bp，编码13个蛋白质编码基因（PCGs）、21个转运RNA基因（tRNAs）和2个核糖体RNA基因。该线粒体基因组的A+T含量为75.11%，具有正的AT偏斜（0.064）和负的GC偏斜（-0.192）。10个PCGs以典型的ATN密码子起始，而其余3个PCGs分别以AAC（）和TTG（和）起始。除缺少二氢尿嘧啶臂外，所有tRNAs均具有典型的二级三叶草结构。基于13个PCGs核苷酸序列的贝叶斯系统发育分析在叶甲科内恢复了一个系统发育关系：（（（叶甲亚科+隐头叶甲亚科），（（（叶甲亚科，亮叶甲亚科），龟甲亚科），叶甲亚科）），豆象亚科）。
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