Zhang Li-Jie, Wu Ling, Wei Chun-Yan, Liu Xing-Liang, Xue Huai-Jun, Yang Xing-Ke, Nie Rui-E
Inspection and Quarantine Technical Center, Beijing Inspection and Quarantine Testing Bureau, Beijing, China.
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Mitochondrial DNA B Resour. 2018 May 26;3(2):645-647. doi: 10.1080/23802359.2017.1413308.
In this study, the complete 17,809 bp mitochondrial genome of (F.) (Coleoptera: Chrysomelidae: Bruchinae) was sequenced using Illumina's HiSeq2000 platform. The mitogenome is a double-stranded circular molecule of 17,809 bp in length with 21 transfer RNA genes, 13 protein-coding genes, and two ribosomal RNA genes as in other insects. Specially, there is a 2008 bp-inserted segment between ND2 and tRNA-Trp from 1180 to 3187, which cannot be aligned to any known gene of mitogenomes. To estimate the taxonomic status of Bruchinae, total 17 species from eight subfamilies of Chrysomelidae were selected as ingroups and three species of Lamiinae as outgroups for phylogenetic analysis based on mitogenome. The results showed that three major lineages were formed, including a basal 'Eumolpine' clade (Cassidinae, Eumolpinae, Cryptocephalinae, Clytrinae), ''Criocerine' clade (Criocerinae, Bruchinae) and 'Chrysomeline' clade (Chrysomelinae, Galerucinae s. l.). Bruchinae showed more closed relationship with Criocerinae than other subfamilies. More thorough taxon sampling will be needed to well understand the relationship in Chrysomelidae.
在本研究中，利用Illumina公司的HiSeq2000平台对（F.）（鞘翅目：叶甲科：豆象亚科）的完整线粒体基因组（17,809 bp）进行了测序。该线粒体基因组是一个长度为17,809 bp的双链环状分子，与其他昆虫一样，含有21个转运RNA基因、13个蛋白质编码基因和两个核糖体RNA基因。特别的是，在ND2和tRNA-Trp之间存在一个2008 bp的插入片段，位置从1180到3187，该片段无法与任何已知的线粒体基因组基因进行比对。为了评估豆象亚科的分类地位，基于线粒体基因组，从叶甲科八个亚科中总共选择了17个物种作为内群，并选择了三个天牛亚科物种作为外群进行系统发育分析。结果表明，形成了三个主要分支，包括一个基部的“真叶甲类”分支（龟甲亚科、真叶甲亚科、隐头叶甲亚科、隐头叶甲亚科）、“叶甲类”分支（叶甲亚科、豆象亚科）和“叶甲科类”分支（叶甲亚科、广义叶甲亚科）。豆象亚科与叶甲亚科的关系比与其他亚科的关系更为密切。需要更全面的分类单元采样，以便更好地了解叶甲科中的关系。
