Wang Jie, Wu Zhi-Qiang, Ma Li, Gu Cui-Hua
School of Landscape and Architecture, Zhejiang A & F University, Hangzhou, China.
Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang A & F University, Hangzhou, China.
Mitochondrial DNA B Resour. 2020 May 14;5(3):2070-2071. doi: 10.1080/23802359.2020.1764401. eCollection 2020.
Pomegranate ( L.) is of great significance both as a fruit tree and an ornamental plant. Hereon, we sequenced and characterized the complete chloroplast genome of 'Nana' and performed phylogenetic analysis concerning related species. It turned out that the length of chloroplast genome sequence reached 158,639 bp and exhibited a four-conjoined structure, i.e., a large single copy region (LSC, 89,022 bp), a small single copy region (SSC, 18,685 bp) and twain inverted repeat regions (IRa and IRb, 25,466 bp). 112 unique genes were identified, consisting of 78 protein-coding genes, four ribosomal RNA (rRNA) genes and 30 transfer RNA (tRNA) genes. The result of phylogenetic analysis based on Neighbor-joining (NJ) method was consistent with that of Bayesian inference (BI), which strongly supported that 'Nana' was close to its original species and they together had a close relationship with within Lythraceae.
石榴（Punica granatum L.）作为一种果树和观赏植物都具有重要意义。在此，我们对‘Nana’的完整叶绿体基因组进行了测序和特征分析，并对相关物种进行了系统发育分析。结果表明，叶绿体基因组序列长度达到158,639 bp，呈现出四分体结构，即一个大单拷贝区域（LSC，89,022 bp）、一个小单拷贝区域（SSC，18,685 bp）和两个反向重复区域（IRa和IRb，25,466 bp）。共鉴定出112个独特基因，包括78个蛋白质编码基因、4个核糖体RNA（rRNA）基因和30个转运RNA（tRNA）基因。基于邻接法（NJ）的系统发育分析结果与贝叶斯推断（BI）的结果一致，有力地支持了‘Nana’与其原种相近，并且它们与千屈菜科内的[具体物种]关系密切。
