下一代测序技术获得了中华鼢鼠的完整线粒体基因组。
Next generation sequencing yields the complete mitogenome of Smith's zokor ().
作者信息
Cai Zhenyuan, Zhang Jingjie, Qiao Penghai, Qing Wen, Zhang Tongzuo
机构信息
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
QinghaiProvincial Key Laboratory of Animal Ecological Genomics, Xining, China.
出版信息
Mitochondrial DNA B Resour. 2020 May 18;5(3):2109-2110. doi: 10.1080/23802359.2020.1765211. eCollection 2020.
Sequencing analysis of mitochondrial genomes is useful for understanding the genome structures. In this study, complete mitochondrial genomes of the was obtained by using next generation sequencing method. The complete mitogenome of was 16,350 bp long, containing 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 1 non-coding control region (D-loop). The overall base composition of the heavy strand is A (33.65%), C (23.80%), T (30.31%), and G (12.24%). The base compositions present highly biased toward A + T nucleotides. The result of phylogenetic analysis showed the five species formed a monophyly with the high bootstrap value and as a sister group of the genus . This is the first report of the complete mitochondrial genomes of and the mitogenome is potentially important for evolutionary biology, population genetics, and species diagnosis studies of the Mysopalacinae.
线粒体基因组的测序分析有助于理解基因组结构。在本研究中，通过使用下一代测序方法获得了[物种名称]的完整线粒体基因组。[物种名称]的完整线粒体基因组长度为16,350 bp，包含13个蛋白质编码基因（PCGs）、2个核糖体RNA（rRNA）基因、22个转运RNA（tRNA）基因和1个非编码控制区（D-loop）。重链的总体碱基组成为A（33.65%）、C（23.80%）、T（30.31%）和G（12.24%）。碱基组成高度偏向于A+T核苷酸。系统发育分析结果表明，这五个[物种名称]物种形成了一个具有高自展值的单系群，并作为[属名]属的姐妹群。这是关于[物种名称]完整线粒体基因组的首次报道，该线粒体基因组对于鼢鼠亚科的进化生物学、群体遗传学和物种诊断研究可能具有重要意义。
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