下一代测序技术获得了沙蚕（环节动物门：沙蚕科）的完整线粒体基因组。
Next generation sequencing yields the complete mitogenome of nereid worm, (Annelida: Nereididae).
作者信息
Lin Geng-Ming, Shen Kang-Ning, Hsiao Chung-Der
机构信息
Laboratory of Marine Biology and Ecology, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.
Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan.
出版信息
Mitochondrial DNA B Resour. 2016 Feb 1;1(1):103-104. doi: 10.1080/23802359.2015.1137845.
In this study, the complete mitogenome sequence of nereid worm, (Annelida: Nereididae), has been decoded for the first time by low coverage genome sequencing method. The overall base composition of mitogenome is 30.8% for A, 24.6% for C, 14.0% for G and 30.6% for T, and has GC content of 38.6%. The assembled mitogenome, consisting of 15 265 bp, has unique 13 protein-coding genes (PCGs), 21 transfer RNAs and two ribosomal RNA genes. The complete mitogenome of shows 69% identities to . All PCGs, tRNA and rRNA genes were encoded on H-strand. The potential D-loop is 827 bp in length and located between tRNA-Gly and tRNA-Trp. The complete mitogenome provides essential and important DNA molecular data for further phylogenetic and evolutionary analysis for Annelida.
在本研究中，沙蚕（环节动物门：沙蚕科）的完整线粒体基因组序列首次通过低覆盖度基因组测序方法得到解码。该线粒体基因组的总体碱基组成为：A占30.8%，C占24.6%，G占14.0%，T占30.6%，GC含量为38.6%。组装后的线粒体基因组由15265个碱基对组成，包含独特的13个蛋白质编码基因（PCGs）、21个转运RNA和两个核糖体RNA基因。该沙蚕的完整线粒体基因组与另一种沙蚕的线粒体基因组有69%的同源性。所有的PCGs、tRNA和rRNA基因都在重链上编码。潜在的D环长度为827个碱基对，位于tRNA-Gly和tRNA-Trp之间。完整的线粒体基因组为环节动物进一步的系统发育和进化分析提供了重要的DNA分子数据。
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