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红树林蕨类植物的完整叶绿体基因组。

The complete chloroplast genomes of the mangrove fern .

作者信息

Zhong Yan, Yu Runxian, Chen Yongmei, Zhou Renchao, Liu Ying

机构信息

State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.

School of Chemical Engineering, Sichuan University of Science & Engineering, Zigong, China.

出版信息

Mitochondrial DNA B Resour. 2020 Jun 1;5(3):2258-2259. doi: 10.1080/23802359.2020.1772140. eCollection 2020.

DOI:10.1080/23802359.2020.1772140
PMID:33366998
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7510633/
Abstract

The mangrove fern is widely distributed in the Indo West-Pacific and Atlantic East-Pacific regions. Here we assembled and annotated its chloroplast genome based on the Illumina sequencing reads. The complete chloroplast genome of was 154,805 bp in length with the GC content of 38.38%. It contains a large single copy (LSC) region of 82,826 bp and a small single copy (SSC) region of 21,617 bp, separated by a pair of inverted repeat region (IRs) of 25,181 bp each. It contains 84 protein coding genes, 27 tRNA genes, and four rRNA genes. Phylogenetic analysis shows that is closest to in the subfamily Parkerioideae. The chloroplast genome of reported here offers a useful resource for its phylogeography and conservation genetics studies.

摘要

红树蕨广泛分布于印度-西太平洋和大西洋东太平洋地区。在此,我们基于Illumina测序读数组装并注释了其叶绿体基因组。其完整的叶绿体基因组长度为154,805 bp,GC含量为38.38%。它包含一个82,826 bp的大单拷贝(LSC)区域和一个21,617 bp的小单拷贝(SSC)区域,由一对各为25,181 bp的反向重复区域(IR)隔开。它包含84个蛋白质编码基因、27个tRNA基因和4个rRNA基因。系统发育分析表明,它在帕克蕨亚科中与[某物种]关系最为密切。此处报道的[该物种]叶绿体基因组为其系统地理学和保护遗传学研究提供了有用的资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d36a/7510633/3e2592b8a387/TMDN_A_1772140_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d36a/7510633/3e2592b8a387/TMDN_A_1772140_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d36a/7510633/3e2592b8a387/TMDN_A_1772140_F0001_B.jpg

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本文引用的文献

1
PGA: a software package for rapid, accurate, and flexible batch annotation of plastomes.PGA:一个用于叶绿体基因组快速、准确且灵活批量注释的软件包。
Plant Methods. 2019 May 21;15:50. doi: 10.1186/s13007-019-0435-7. eCollection 2019.
2
NOVOPlasty: de novo assembly of organelle genomes from whole genome data.NOVOPlasty:从头组装细胞器基因组的全基因组数据。
Nucleic Acids Res. 2017 Feb 28;45(4):e18. doi: 10.1093/nar/gkw955.
3
Transcriptome analyses provide insights into the phylogeny and adaptive evolution of the mangrove fern genus Acrostichum.
转录组分析为红树林蕨类植物芒萁属的系统发育和适应性进化提供了见解。
Sci Rep. 2016 Oct 26;6:35634. doi: 10.1038/srep35634.
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RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies.RAxML 版本 8:用于系统发育分析和大型系统发育后分析的工具。
Bioinformatics. 2014 May 1;30(9):1312-3. doi: 10.1093/bioinformatics/btu033. Epub 2014 Jan 21.
5
OrganellarGenomeDRAW--a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets.细胞器基因组绘图软件--一套用于生成质体和线粒体基因组物理图谱以及可视化表达数据集的工具。
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W575-81. doi: 10.1093/nar/gkt289. Epub 2013 Apr 22.
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MAFFT multiple sequence alignment software version 7: improvements in performance and usability.MAFFT 多序列比对软件版本 7:性能和易用性的改进。
Mol Biol Evol. 2013 Apr;30(4):772-80. doi: 10.1093/molbev/mst010. Epub 2013 Jan 16.