Choi Sang Chul, Kim Sangtae
Department of Biotechnology, Sungshin University, Seoul, Korea.
Mitochondrial DNA B Resour. 2020 Nov 13;5(3):3743-3745. doi: 10.1080/23802359.2020.1834889.
(Nakai) Nakai is an endemic species in Korea. We present the second complete chloroplast genome sequence of showing that the chloroplast genome is 156,376 bp in length containing four subregions: a large single-copy (LSC) region of 87,097 bp, a small single-copy (SSC) region of 17,859 bp, and a pair of inverted repeat regions (IRs) of 25,710 bp. The genome contains 131 genes including 88 protein-coding genes, 35 s, and eight rRNAs. We found 40 base differences in 17 sites between our chloroplast genome and the previously reported chloroplast genome sequences of . All of the differences were found in a region of 832 bp in the LSC region except for one site, which indicated potential sequencing errors in building the previously reported genome because of little substitutions in the homologous region of the other species and even of , a sister to . The new chloroplast genomes of and those of and are identical, which suggests that is maternally closely related to the other two species.
（中井）中井是韩国的特有物种。我们展示了第二个完整的叶绿体基因组序列，表明叶绿体基因组长度为156,376 bp，包含四个亚区域：一个87,097 bp的大单拷贝（LSC）区域、一个17,859 bp的小单拷贝（SSC）区域以及一对25,710 bp的反向重复区域（IRs）。该基因组包含131个基因，包括88个蛋白质编码基因、35个tRNA和8个rRNA。我们发现我们的叶绿体基因组与先前报道的[物种名称]叶绿体基因组序列在17个位点存在40个碱基差异。除一个位点外，所有差异均在LSC区域的一个832 bp区域中发现，这表明在构建先前报道的基因组时可能存在测序错误，因为在其他[物种名称]甚至其姐妹种[物种名称]的同源区域中替换很少。[物种名称]的新叶绿体基因组与[物种名称]和[物种名称]的相同，这表明[物种名称]在母系上与其他两个物种密切相关。
