Brazilian Nanotechnology National Laboratory (LNNano), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Sao Paulo, Brazil.
"Gleb Wataghim" Institute of Physics (IFGW), University of Campinas (Unicamp), Campinas, Sao Paulo, Brazil.
J Mater Chem B. 2021 Jan 14;9(2):428-439. doi: 10.1039/d0tb02454f. Epub 2020 Dec 8.
The use of hybrid nanostructures based on magneto-luminescent properties is a promising strategy for nano-bio applications and theranostics platforms. In this work, we carried out the synthesis and functionalization of iron oxide nanocubes (IONCs) to obtain multifunctional hybrid nanostructures towards biomedical applications. The IONCs were functionalized with tetraethylorthosilicate, thenoyltrifluoroacetone-propyl-triethoxysilane and europium(iii)-dibenzoylmethane complexes to obtain the materials termed as IOCNCs@SiO, IONCs@SiOTTA, IONCs@SiOTTA-Eu and IONCs@SiO-TTA-Eu-DBM, respectively. Then, the biological interactions of these nanostructures with red blood cells - RBCs (hemolysis) and human blood plasma (protein corona formation) were evaluated. The XPS spectrocopy and EDS chemical mapping analysis showed that each domain is homogeneously occupied in the hybrid material, with the magnetic core at the center and the luminescent domain on the surface of the hybrid nanomaterial with a core@shell like structure. Futhermore, after each functionalization step, the nanomaterial surface charge drastically changed, with critical impact on RBC lysis and corona formation. While IONCs@SiO and IONCs@SiO-TTA-Eu-DBM showed hemolytic properties in a dose-dependent manner, the IONCs@SiOTTA-Eu did not present any hemolytic effect up to 300 μg mL. Protein corona results showed a pattern of selective adsorption of proteins with each surface of the synthesized hybrid materials. However, as a general result, a suppression of hemolysis after protein corona formation in all tests was verified. Finally, this study provides a solid background for further applications of these hybrid magneto-luminescent materials containing new surface functionalities in the emerging field of medical nanobiotechnology.
基于磁致发光性质的杂化纳米结构的应用是纳米生物应用和治疗学平台的一种有前途的策略。在这项工作中，我们进行了氧化铁纳米立方（IONC）的合成和功能化，以获得用于生物医学应用的多功能杂化纳米结构。IONC 用四乙氧基硅烷、三氟乙酰丙酮丙基三乙氧基硅烷和铕（III）-二苯甲酰甲烷配合物进行功能化，分别得到材料 IOCNCs@SiO、IONCs@SiOTTA、IONCs@SiOTTA-Eu 和 IONCs@SiO-TTA-Eu-DBM。然后，评估了这些纳米结构与红细胞（溶血）和人血浆（蛋白质冠形成）的生物相互作用。XPS 光谱和 EDS 化学映射分析表明，每个域均匀地占据在杂化材料中，磁性核位于中心，发光域位于杂化纳米材料的表面，具有核@壳结构。此外，在每个功能化步骤之后，纳米材料的表面电荷发生了剧烈变化，对 RBC 溶血和冠形成有重大影响。虽然 IONCs@SiO 和 IONCs@SiO-TTA-Eu-DBM 表现出剂量依赖性的溶血性质，但 IONCs@SiOTTA-Eu 直到 300μg/mL 也没有表现出任何溶血作用。蛋白质冠的结果显示，合成杂化材料的每个表面都具有选择性吸附蛋白质的模式。然而，作为一般结果，在所有测试中，在形成蛋白质冠后都证实了溶血的抑制。最后，本研究为这些含有新表面功能的新型磁致发光杂化材料在新兴的医学纳米生物技术领域的进一步应用提供了坚实的基础。
