Castellanos-Rubio Idoia, Barón Ander, Luis-Lizarraga Oier, Rodrigo Irati, de Muro Izaskun Gil, Orue Iñaki, Martínez-Martínez Virginia, Castellanos-Rubio Ainara, López-Arbeloa Fernando, Insausti Maite
Departamento Química Orgánica e Inorgánica, Facultad de Ciencia y Tecnología, UPV/EHU, Barrio Sarriena s/n, 48940, Leioa, Spain.
Departamento Electricidad y Electrónica, Facultad de Ciencia y Tecnología, UPV/EHU, Barrio Sarriena s/n, Leioa48940, Spain.
ACS Appl Mater Interfaces. 2022 Oct 27;14(44):50033-44. doi: 10.1021/acsami.2c14016.
Nanosystems that simultaneously contain fluorescent and magnetic modules can offer decisive advantages in the development of new biomedical approaches. A biomaterial that enables multimodal imaging and contains highly efficient nanoheaters together with an intrinsic temperature sensor would become an archetypical theranostic agent. In this work, we have designed a magneto-luminescent system based on FeO NPs with large heating power and thermosensitive rhodamine (Rh) fluorophores that exhibits the ability to self-monitor the hyperthermia degree. Three samples composed of highly homogeneous FeO NPs of ∼25 nm and different morphologies (cuboctahedrons, octahedrons, and irregular truncated-octahedrons) have been finely synthesized. These NPs have been thoroughly studied in order to choose the most efficient inorganic core for magnetic hyperthermia under clinically safe radiofrequency. Surface functionalization of selected FeO NPs has been carried out using fluorescent copolymers composed of PMAO, PEG and Rh. Copolymers with distinct PEG tail lengths (5-20 kDa) and different Rh percentages (5, 10, and 25%) have been synthesized, finding out that the copolymer with 20 kDa PEG and 10% Rh provides the best coating for an efficient fluorescence with minimal aggregation effects. The optimized FeO@Rh system offers very suitable fluorescence thermosensitivity in the therapeutic hyperthermia range. Additionally, this sample presents good biocompatibility and displays an excellent heating capacity within the clinical safety limits of the AC field (≈ 1000 W/g at 142 kHz and 44 mT), which has been confirmed by both calorimetry and AC magnetometry. Thus, the current work opens up promising avenues toward next-generation medical technologies.
同时包含荧光和磁性模块的纳米系统在新型生物医学方法的开发中可提供决定性优势。一种能够实现多模态成像且包含高效纳米加热器以及内在温度传感器的生物材料将成为典型的治疗诊断试剂。在这项工作中，我们设计了一种基于具有大加热功率的FeO纳米颗粒和热敏罗丹明（Rh）荧光团的磁光系统，该系统具有自我监测热疗程度的能力。精细合成了由约25nm高度均匀的FeO纳米颗粒和不同形态（立方八面体、八面体和不规则截角八面体）组成的三个样品。为了在临床安全的射频下选择用于磁热疗的最有效无机核心，对这些纳米颗粒进行了深入研究。使用由PMAO、PEG和Rh组成的荧光共聚物对选定的FeO纳米颗粒进行了表面功能化。合成了具有不同PEG尾长（5-20kDa）和不同Rh百分比（5%、10%和25%）的共聚物，发现具有20kDa PEG和10% Rh的共聚物提供了最佳涂层，可实现高效荧光且聚集效应最小。优化后的FeO@Rh系统在治疗热疗范围内具有非常合适的荧光热敏感性。此外，该样品具有良好的生物相容性，并且在交流场的临床安全极限内（在142kHz和44mT下约为1000W/g）表现出优异的加热能力，这已通过量热法和交流磁强计得到证实。因此，当前的工作为下一代医疗技术开辟了有前景的途径。
