基于半花菁染料和恶唑烷开关的近红外荧光探针用于检测线粒体 pH 值。
A near-infrared fluorescent probe based on a hemicyanine dye with an oxazolidine switch for mitochondrial pH detection.
机构信息
Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA.
出版信息
J Mater Chem B. 2021 Jan 28;9(3):857-863. doi: 10.1039/d0tb02181d.
Abstract
A near-infrared fluorescent probe (AH+) has been prepared by incorporating an oxazolidine switch into a near-infrared hemicyanine dye. The probe shows fast and sensitive responses to pH from an oxazolidine switch to the hemicyanine dye upon pH decreases from 10.0 to 5.0. The probe shows good photostability, low cytotoxicity, and reversible fluorescence responses to pH changes with a pKa value of 7.6. It has been successfully used to determine pH changes in mitochondria.
摘要
一种近红外荧光探针（AH+）是通过将恶唑烷开关整合到近红外半花菁染料中制备的。该探针在 pH 值从 10.0 降低到 5.0 时，通过恶唑烷开关到半花菁染料的快速和灵敏响应显示出对 pH 的快速和灵敏响应。该探针具有良好的光稳定性、低细胞毒性和对 pH 值变化的可逆荧光响应，pKa 值为 7.6。它已成功用于测定线粒体中的 pH 值变化。
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