Cragnolini Tristan, Sweeney Aaron, Topf Maya
Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck College, University of London, London, UK.
Methods Mol Biol. 2021;2215:189-223. doi: 10.1007/978-1-0716-0966-8_9.
The resolving power of cryo-EM experiments has dramatically improved in recent years. However, many cryo-EM maps may still not achieve a resolution that is sufficiently high to allow model building directly from the map. Instead, it is common practice to fit an initial atomic model to the map and refine this model. Depending on the resolution and whether the structure suffers from inherent flexibility or experimental limitations, different methods can be applied, to obtain high-quality, well-fitted atomic model of the macromolecular assembly represented by the map, and to assess its properties. In this review, we describe some of these methods, with the main focus on those that have been developed in our group over the last decade.
近年来，冷冻电镜实验的分辨率有了显著提高。然而，许多冷冻电镜图谱的分辨率可能仍不足以直接从图谱构建模型。相反，通常的做法是将初始原子模型拟合到图谱上并对该模型进行优化。根据分辨率以及结构是否存在固有柔性或实验限制，可以应用不同的方法，以获得由图谱表示的大分子组装体的高质量、拟合良好的原子模型，并评估其性质。在本综述中，我们描述了其中一些方法，主要侧重于过去十年中我们团队开发的那些方法。
