用于微 ED 的连续旋转数据采集自动化。

Automation of Continuous-Rotation Data Collection for MicroED.

机构信息

Structural Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

出版信息

Methods Mol Biol. 2021;2215:321-327. doi: 10.1007/978-1-0716-0966-8_16.

Abstract

Automated coordination of microscope and camera functions for MicroED data collection simplifies the procedure for robust dataset acquisition and enables unattended sequential collection of many crystal targets. This chapter discusses the prerequisites for an algorithm of data collection automation for continuous-rotation MicroED and presents a practical protocol for achieving this goal using the popular TEM control software program SerialEM.

摘要

自动化协调显微镜和相机功能进行 MicroED 数据采集简化了稳健数据集采集的过程，并能够实现无人值守的多个晶体目标连续采集。本章讨论了连续旋转 MicroED 数据采集自动化算法的前提条件，并使用流行的 TEM 控制软件程序 SerialEM 介绍了实现这一目标的实用方案。