低温电子显微镜方法——微晶体电子衍射（MicroED）。
The cryo-EM method microcrystal electron diffraction (MicroED).
机构信息
Chemical Engineering, School for Engineering of Matter, Transport, and Energy, Arizona State University, Tempe, AZ, USA.
Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, USA.
出版信息
Nat Methods. 2019 May;16(5):369-379. doi: 10.1038/s41592-019-0395-x. Epub 2019 Apr 29.
Abstract
In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal electron diffraction (MicroED). Since that time, data collection and analysis schemes have been fine-tuned, and structures for more than 40 different proteins, oligopeptides and organic molecules have been determined. Here we review the MicroED technique and place it in context with other structure-determination methods. We showcase example structures solved by MicroED and provide practical advice to prospective users.
摘要
2013 年，我们建立了一种称为微晶电子衍射（MicroED）的冷冻电子显微镜（cryo-EM）技术。从那时起，数据收集和分析方案已经过微调，已经确定了 40 多种不同蛋白质、寡肽和有机分子的结构。在这里，我们回顾了 MicroED 技术，并将其置于与其他结构测定方法的背景下。我们展示了通过 MicroED 解决的示例结构，并为潜在用户提供了实用建议。
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