Rastak Infertility Center, Arak, Iran.
Department of Midwifery, Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran.
Andrologia. 2021 Mar;53(2):e13932. doi: 10.1111/and.13932. Epub 2020 Dec 25.
This in vitro study aimed to measure the ameliorative effect of L-carnitine against apoptosis, DNA fragmentation, membrane integrity and lipid peroxidation of spermatozoa from men with asthenoteratozoospermia (ATS). L-carnitine has an impressive effect on boosting the quality and quantity of spermatozoa and also can prevent apoptosis induction. For this purpose, semen samples were collected from 50 ATS men. Semen was divided into control and L-carnitine (0.5 mg/ml) groups at 2, 4, 6 and 24 hr. Concentrating on the reasons for apoptosis is an arduous process, but in the present research for this evaluation, mitochondrial membrane potential (MMP), DNA fragmentation by TUNEL and SCD methods, and lipid peroxidation were carried out. Also, sperm viability was performed. In the control group, MDA levels were increased significantly at 6 hr; however, sperm viability was decreased significantly at 4 and 6 hr. Moreover, in the L-carnitine group, TUNEL, SCD and MDA levels were decreased significantly and MMP and viability were increased significantly compared with the control group. In this writers' view, in vitro L-carnitine treatment can downregulate apoptosis in men with ATS.
本体外研究旨在测量左卡尼汀对弱精子症（ATS）患者精子凋亡、DNA 碎片化、膜完整性和脂质过氧化的改善作用。左卡尼汀对提高精子的质量和数量有显著的效果，并且可以预防细胞凋亡的诱导。为此，从 50 名 ATS 男性中采集精液样本。精液在 2、4、6 和 24 小时时分为对照组和左卡尼汀（0.5mg/ml）组。关注凋亡的原因是一个艰巨的过程，但在本研究中，我们通过线粒体膜电位（MMP）、TUNEL 和 SCD 方法检测 DNA 碎片化以及脂质过氧化来评估凋亡。此外，还进行了精子活力检测。在对照组中，MDA 水平在 6 小时时显著增加；然而，精子活力在 4 和 6 小时时显著下降。此外，与对照组相比，左卡尼汀组的 TUNEL、SCD 和 MDA 水平显著降低，MMP 和活力显著增加。在本研究中，体外左卡尼汀处理可以下调 ATS 患者的细胞凋亡。
