Genomics Group, Department of Genetics, University of São Paulo/Luiz de Queiroz College of Agriculture (USP/ESALQ), Piracicaba SP 13418-900, Brazil.
Mendelics Análise Genômica S.A., São Paulo SP 04013-000, Brazil.
Mol Plant Microbe Interact. 2021 Apr;34(4):448-452. doi: 10.1094/MPMI-08-20-0218-A. Epub 2021 Mar 24.
Here, we present the first complete chromosome-level genome assembly of the smut fungus strain SPL10A, the causal agent of the sourgrass () smut disease. Combining Illumina paired-end and Nanopore long reads, we generated a final assembly composed of 23 chromosomes (22 nuclear and one mitochondrial) with 18,915,934 bp. Gene prediction accomplished using extrinsic evidence from the sugarcane smut fungus originated a total of 6,402 protein-encoding genes. The secretome (388 proteins) and the effectorome repertoires (68 candidates) were also predicted, given their crucial roles in plant-pathogen interactions. The complete telomere-to-telomere chromosome sequences of this poorly studied fungus will provide a valuable resource for future comparative genomic studies among smuts to unravel their underlying pathogenicity mechanisms.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
在这里，我们呈现了导致酸草（）黑粉病的黑粉菌菌株 SPL10A 的首个完整染色体水平的基因组组装。我们结合 Illumina 配对末端和纳米孔长读长，生成了一个由 23 条染色体（22 条核染色体和 1 条线粒体染色体）组成的最终组装体，总长度为 18,915,934bp。使用来自甘蔗黑粉菌的外源证据进行基因预测，总共产生了 6,402 个编码蛋白的基因。鉴于其在植物-病原体相互作用中的关键作用，还预测了该真菌的分泌组（388 种蛋白）和效应子组库（68 个候选物）。对这种研究较少的真菌进行完整的端粒到端粒染色体测序，将为黑粉菌之间的未来比较基因组研究提供有价值的资源，以揭示它们潜在的致病性机制。
