Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
Med Mycol. 2021 Jul 6;59(7):683-693. doi: 10.1093/mmy/myaa097.
The genus Malassezia is part of the normal skin mycobiota of a wide range of warm-blooded animals. In this genus, M. cuniculi is the only species described from rabbits. However, Malassezia species are rarely studied in lagomorphs. In the present study, the presence of Malassezia was assessed in samples from the external ear canal of healthy rabbits of different breeds. Cytological and culture techniques, Sanger sequencing, and Next-generation sequencing (NGS) were used to describe the ear mycobiota in the samples. Although no growth was observed in the cultured plates, cytological examination revealed the presence of round cells similar to those of Malassezia yeasts. For metagenomics analysis, the D1/D2 domain of the large subunit of the ribosomal DNA (LSU rDNA) was PCR amplified and the resulting reads were mapped against a custom-made cured database of 26S fungal sequences. NGS analysis revealed that Basidiomycota was the most abundant phylum in all the samples followed by Ascomycota. Malassezia was the most common genus presenting the highest abundance in the external ear canal. Malassezia phylotype 131 and M. cuniculi were the main sequences detected in the external auditory canal of rabbits. The study included both lop-eared and erect-eared rabbits and no differences were observed in the results when comparing both groups. This is the first attempt to study the external ear canal mycobiome of rabbits of different breeds using NGS.
In the present study, the presence of Malassezia was assessed in samples from the external ear canal of healthy rabbits of different breeds. Cytological and culture techniques, Sanger sequencing, and Next-generation sequencing (NGS) were used to describe the ear mycobiota in the samples.
马拉色菌属是广泛的温血动物正常皮肤真菌区系的一部分。在该属中，只有从兔子中描述的 M. cuniculi 一种物种。然而，马拉色菌在兔类中很少被研究。在本研究中，评估了来自不同品种健康兔的外耳耳道样本中马拉色菌的存在。细胞学和培养技术、Sanger 测序和下一代测序（NGS）用于描述样本中的耳部真菌群。尽管在培养平板上未观察到生长，但细胞学检查显示存在类似于马拉色菌酵母的圆形细胞。对于宏基因组学分析，扩增了核糖体 DNA（LSU rDNA）大亚基的 D1/D2 结构域，并且将所得读取序列与定制的 26S 真菌序列 cured 数据库进行比对。NGS 分析表明，在所有样本中，担子菌门是最丰富的门，其次是子囊菌门。马拉色菌是最常见的属，在外耳道中丰度最高。在外耳道中检测到的马拉色菌 131 型和 M. cuniculi 是主要序列。该研究包括垂耳兔和立耳兔，比较两组时，结果没有差异。这是首次尝试使用 NGS 研究不同品种兔的外耳耳道真菌组。
在本研究中，评估了来自不同品种健康兔的外耳耳道样本中马拉色菌的存在。细胞学和培养技术、Sanger 测序和下一代测序（NGS）用于描述样本中的耳部真菌群。
