健康奶牛外耳道的微生物组。

Mycobiome of the external ear canal of healthy cows.

机构信息

Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.

Sequentia Biotech SL, Barcelona, Catalonia, Spain.

出版信息

Med Mycol. 2024 May 3;62(5). doi: 10.1093/mmy/myae049.

DOI:10.1093/mmy/myae049

PMID:38684473

Abstract

Malassezia yeasts belong to the normal skin microbiota of a wide range of warm-blooded animals. However, their significance in cattle is still poorly understood. In the present study, the mycobiota of the external ear canal of 20 healthy dairy Holstein cows was assessed by cytology, culture, PCR, and next-generation sequencing. The presence of Malassezia was detected in 15 cows by cytology and PCR. The metagenomic analysis revealed that Ascomycota was the predominant phylum but M. pachydermatis the main species. The Malassezia phylotype 131 was detected in low abundance. Nor M. nana nor M. equina were detected in the samples.

摘要

马拉色菌酵母属于多种温血动物正常皮肤微生物群。然而，它们在牛中的意义仍知之甚少。在本研究中，通过细胞学、培养、PCR 和下一代测序评估了 20 头健康荷斯坦奶牛外耳耳道的真菌群。细胞学和 PCR 检测到 15 头奶牛存在马拉色菌。宏基因组分析显示，子囊菌门是主要的门，但厚皮马拉色菌是主要的种。低丰度检测到马拉色菌 131 型。样本中未检测到 nor M. nana 或 nor M. equina。