Department of Second Cardiology, Affiliated Hospital of Hebei University, Baoding, Hebei, People's Republic of China.
Department of Cardiology, Xushui Huayi hospital, Baoding, Hebei, People's Republic of China.
Biotechnol Appl Biochem. 2022 Feb;69(1):166-171. doi: 10.1002/bab.2093. Epub 2021 Jan 17.
Myocardial infarction (MI) is considered as one of the major life-threatening health issues worldwide. Growing number of cases every year is demanding rapid, portable, and early detection by the sensing devices for the identification of MI. This research work introduces a modified interdigitated electrode (IDE) sensing surface constructed with single-walled carbon nanotube (SWCN) to detect the cardiac biomarker, C-reactive protein (CRP). CRP-specific aptamer was conjugated with gold nanoparticle and attached on SWCN-constructed IDE surface. This probe-modified sensing surface has reached the limit of CRP detection to 10 pM on a linear regression curve with the regression coefficient of R² = 0.9223 [y = 0.9198x - 0.4326]. Further, control molecules, such as random aptamer sequence and nontarget cardiac biomarker (Troponin I), did not show the current response, indicating the specific CRP detection. This sensing strategy helps to detect the lower level of CRP and diagnose the MI at its earlier stages.
心肌梗死（MI）被认为是全球范围内威胁生命的主要健康问题之一。每年病例数量的增加要求传感设备快速、便携和早期检测，以识别 MI。这项研究工作介绍了一种改进的叉指电极（IDE）传感表面，由单壁碳纳米管（SWCN）构建，用于检测心脏生物标志物 C 反应蛋白（CRP）。CRP 特异性适体与金纳米颗粒缀合，并附着在 SWCN 构建的 IDE 表面上。在一条线性回归曲线上，该探针修饰的传感表面已经达到了 CRP 检测的极限，为 10 pM，其回归系数 R²=0.9223 [y=0.9198x-0.4326]。此外，对照分子，如随机适体序列和非靶标心脏生物标志物（肌钙蛋白 I），没有显示出电流响应，表明可以特异性检测 CRP。这种传感策略有助于检测到更低水平的 CRP，并在早期阶段诊断心肌梗死。
