Hui Hu, Gopinath Subash C B, Ismail Zool H, Chen Yeng, Pandian K, Velusamy Palaniyandi
Department of Geriatrics, Luzhou People's Hospital, Luzhou, China.
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), Kangar, Perlis, 01000, Malaysia.
Biotechnol Appl Biochem. 2023 Apr;70(2):581-591. doi: 10.1002/bab.2380. Epub 2022 Jul 14.
Myocardial infarction (MI) is highly related to cardiac arrest leading to death and organ damage. Radiological techniques and electrocardiography have been used as preliminary tests to diagnose MI; however, these techniques are not sensitive enough for early-stage detection. A blood biomarker-based diagnosis is an immediate solution, and due to the high correlation of troponin with MI, it has been considered to be a gold-standard biomarker. In the present research, the cardiac biomarker troponin I (cTnI) was detected on an interdigitated electrode sensor with various surface interfaces. To detect cTnI, a capture aptamer-conjugated gold nanoparticle probe and detection antibody probe were utilized and compared through an alternating sandwich pattern. The surface metal oxide morphology of the developed sensor was proven by microscopic assessments. The limit of detection with the aptamer-gold-cTnI-antibody sandwich pattern was 100 aM, while it was 1 fM with antibody-gold-cTnI-aptamer, representing 10-fold differences. Further, the high performance of the sensor was confirmed by selective cTnI determination in serum, exhibiting superior nonfouling. These methods of determination provide options for generating novel assays for diagnosing MI.
心肌梗死（MI）与导致死亡和器官损伤的心脏骤停高度相关。放射技术和心电图已被用作诊断MI的初步检查；然而，这些技术对早期检测的敏感性不够。基于血液生物标志物的诊断是一个直接的解决方案，由于肌钙蛋白与MI的高度相关性，它被认为是一种金标准生物标志物。在本研究中，在具有各种表面界面的叉指电极传感器上检测心脏生物标志物肌钙蛋白I（cTnI）。为了检测cTnI，使用了捕获适体缀合的金纳米颗粒探针和检测抗体探针，并通过交替夹心模式进行比较。通过显微镜评估证实了所开发传感器的表面金属氧化物形态。适体-金-cTnI-抗体夹心模式的检测限为100 aM，而抗体-金-cTnI-适体模式的检测限为1 fM，相差10倍。此外，通过在血清中选择性测定cTnI证实了该传感器的高性能，表现出优异的抗污染性能。这些测定方法为生成用于诊断MI的新型检测方法提供了选择。
