Zincke Diansy, Norris Michael H, Cruz Odalis, Kurmanov Berzhan, McGraw W Scott, Daegling David J, Krigbaum John, Hoang Thi Thu Ha, Khanipov Kamil, Golovko Georgiy, Hadfield Ted, Blackburn Jason K
Spatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida, Gainesville, FL 32611, USA.
Emerging Pathogens Institute, University of Florida, Gainesville, FL 32611, USA.
Pathogens. 2020 Dec 21;9(12):1074. doi: 10.3390/pathogens9121074.
Anthrax is a worldwide zoonotic disease caused by the spore-forming bacterium . Primarily a disease of herbivores, human infections often result from direct contact with contaminated animal products (cutaneous and inhalational anthrax) or through consumption of infected meat (gastrointestinal anthrax). The genetic near neighbor, biovar (Bcbva), causes an anthrax-like illness in the wildlife and livestock of west and central Africa due to the presence and expression of -specific virulence factors in this background. While Bcbva infections have not been reported in humans, a recent seroprevalence study detected Bcbva antibodies in the rural population around Taï National Park. This work describes the development of new TaqMan multiplex PCRs for the simultaneous detection of and Bcbva. The assays are designed to amplify Ba-1, , and markers in and genomic island IV (GI4), , and in Bcbva. Our assays allow for the rapid discrimination of and Bcbva and will provide insights into the molecular epidemiology of these two important pathogens that share an overlapping geographical range in west and central Africa.
炭疽是一种由形成芽孢的细菌引起的全球性人畜共患病。主要是食草动物的疾病，人类感染通常是由于直接接触受污染的动物产品（皮肤炭疽和吸入性炭疽）或通过食用受感染的肉类（胃肠型炭疽）所致。其遗传近邻，生物变种（Bcbva），由于在此背景下存在并表达特定的毒力因子，在西非和中非的野生动物和家畜中引发类似炭疽的疾病。虽然尚未有人类感染Bcbva的报告，但最近一项血清流行率研究在塔伊国家公园周边农村人口中检测到了Bcbva抗体。这项工作描述了用于同时检测和Bcbva的新型TaqMan多重PCR的开发。这些检测方法旨在扩增炭疽杆菌（Ba-1）、、和炭疽杆菌基因组岛IV（GI4）中的标记物，以及Bcbva中的、和。我们的检测方法能够快速区分炭疽杆菌和Bcbva，并将为这两种在西非和中非地理范围重叠的重要病原体的分子流行病学提供见解。
