Thomas G B, Sbarra A J, Feingold M, Cetrulo C L, Shakr C, Newton E, Selvaraj R J
Department of Medical Research, St. Margaret's Hospital for Women, Boston, MA 02125.
Am J Obstet Gynecol. 1988 Jan;158(1):16-22. doi: 10.1016/0002-9378(88)90767-3.
Amniotic fluids obtained by amniocentesis at 16 weeks to term were examined for the presence of Chlamydia trachomatis, Mycoplasma hominis, and Ureaplasma urealyticum. Of 140 fluids tested, none harbored chlamydiae, and only one harbored mycoplasma, M. hominis. A number of amniotic fluids were subsequently tested for their ability to inhibit the growth of these microorganisms. Amniotic fluids and chlamydial suspensions in a 2:1 ratio were incubated 30 to 90 minutes before their inoculation in McCoy cells. Procedures were followed for chlamydial isolation. Genital mycoplasmas were incubated with amniotic fluid samples for 24 or 48 hours at 35 degrees C. Growth in amniotic fluid specimens was compared with growth in pseudoamniotic fluid and broth controls. Fourteen amniotic fluid specimens collected from gestations of 16 to 40 weeks, were found to be inhibitory to the formation of inclusions of C. trachomatis in McCoy cells. Ten amniotic fluid specimens (16 to 39 weeks, gestation) demonstrated various degrees of inhibition against M. hominis, and three fluids were inhibitory to the growth of Ureaplasma. The inhibitor was heat and protease resistant and activity was proportional to concentration. The molecular weight of the inhibitor was probably greater than 10,000 daltons, and pH, although perhaps a contributing factor, was not the cause of the inhibition.
对孕16周直至足月时通过羊膜穿刺术获取的羊水进行检测，以确定是否存在沙眼衣原体、人型支原体和解脲脲原体。在检测的140份羊水中，没有一份含有衣原体，只有一份含有支原体，即人型支原体。随后对一些羊水抑制这些微生物生长的能力进行了检测。羊水与衣原体悬液按2:1的比例在接种到 McCoy 细胞之前孵育30至90分钟。按照衣原体分离的程序进行操作。将生殖道支原体与羊水样本在35℃下孵育24或48小时。将羊水标本中的生长情况与假羊水和肉汤对照中的生长情况进行比较。从孕16至40周收集的14份羊水标本被发现可抑制 McCoy 细胞中沙眼衣原体包涵体的形成。10份羊水标本（孕16至39周）对人型支原体表现出不同程度的抑制作用，3份羊水对解脲脲原体的生长有抑制作用。该抑制剂耐热且耐蛋白酶，其活性与浓度成正比。抑制剂的分子量可能大于10,000道尔顿，pH值虽然可能是一个影响因素，但不是抑制作用的原因。
