单细胞转录组学分析显示分娩期间蜕膜基质细胞存在功能异质性。
Single-cell transcriptomics analysis showing functional heterogeneity in decidual stromal cells during labor.
作者信息
Huang Jingrui, Xie Yingming, Peng Qiaozhen, Wang Weinan, Pei Chenlin, Zhao Yanhua, Liu Rong, Huang Lihui, Li Tieping, Nie Jia, Liu Lijuan, Zhang Xiaowen, Luo Xianggui, Luo Jiefeng, Zhang Weishe
机构信息
Department of Obstetrics, Xiangya Hospital Central South University, Changsha, Hunan, China.
Department of Obstetrics and Gynecology, Changsha Hospital for Maternal and Child Health Care, Changsha, Hunan, China.
出版信息
J Investig Med. 2020 Dec 28;69(4):851-6. doi: 10.1136/jim-2020-001616.
To investigate the heterogeneity of decidual stromal cells (DSCs) and their functional alterations during delivery, we conducted single-cell RNA sequencing analysis to characterize the transcriptomic profiles of DSCs before and after labor onset. According to their transcriptomic profiles, DSCs (6382 cells) were clustered into five subgroups with different functions. Similar to stromal cells, cells in cluster 1 were involved in cell substrate adhesion. On the other hand, cells in clusters 2 and 3 were enriched in signal transduction-related genes. Labor onset led to significant alterations in many pathways, including the activator protein 1 pathway (all clusters), as well as in the response to lipopolysaccharide (clusters 1-3). The downregulated genes were involved in coagulation, ATP synthesis, and oxygen homeostasis, possibly reflecting the oxygen and energy balance during delivery. Our findings highlight that peripartum DSCs are heterogeneous and play multiple roles in labor.
为了研究分娩期间蜕膜基质细胞（DSCs）的异质性及其功能变化，我们进行了单细胞RNA测序分析，以表征分娩发动前后DSCs的转录组图谱。根据其转录组图谱，DSCs（6382个细胞）被聚类为五个具有不同功能的亚组。与基质细胞相似，簇1中的细胞参与细胞与底物的黏附。另一方面，簇2和簇3中的细胞富含信号转导相关基因。分娩发动导致许多信号通路发生显著变化，包括激活蛋白1信号通路（所有簇），以及对脂多糖的反应（簇1-3）。下调的基因涉及凝血、ATP合成和氧稳态，这可能反映了分娩期间的氧和能量平衡。我们的研究结果表明，围产期DSCs具有异质性，在分娩过程中发挥多种作用。
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