Department of Neurosurgery, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
IUBMB Life. 2021 Feb;73(2):398-407. doi: 10.1002/iub.2436. Epub 2020 Dec 28.
Glioblastoma multiforme (GBM) is among the most common adult brain tumors with invariably fatal character. Following the limited conventional therapies, almost all patients, however, presented with symptoms at the time of recurrence. It is dire to develop novel therapeutic strategies to improve the current treatment of GBM. Gallic acid is a well-established antioxidant, presenting a promising new selective anti-cancer drug, while gold nanoparticles (GNPs) can be developed as versatile nontoxic carriers for anti-cancer drug delivery. Here, we prepared gallic acid-GNPs (GA-GNPs) by loading gallic acid onto GNPs, reduction products of tetrachloroauric acid by sodium citrate, through physical and agitation adsorption. GA-GNPs, rather than GNPs alone, significantly inhibited the survival of U251 GBM cells, as well as enhanced radiation-induced cell death. Moreover, GA-GNPs plus radiation arrested the cell cycle of U251 at the S and G2/M phases and triggered apoptotic cell death, which is supported by increased BAX protein levels and decreased expression of BCL-2. Thus, GA-GNPs have great potential in the combination with radiation therapy in future studies for GBM treatment.
多形性胶质母细胞瘤（GBM）是最常见的成人脑肿瘤之一，具有不可避免的致命特征。在有限的传统治疗方法之后，然而，几乎所有患者在复发时都出现了症状。迫切需要开发新的治疗策略来改善目前 GBM 的治疗方法。没食子酸是一种成熟的抗氧化剂，作为一种有前途的新型选择性抗癌药物，而金纳米粒子（GNPs）可以作为多功能无毒的抗癌药物载体进行开发。在这里，我们通过物理和搅拌吸附，将没食子酸加载到 GNPs 上，GNPs 是由柠檬酸三钠还原四氯金酸得到的。GA-GNPs，而不是单独的 GNPs，显著抑制了 U251 GBM 细胞的存活，并增强了辐射诱导的细胞死亡。此外，GA-GNPs 加辐射使 U251 的细胞周期停滞在 S 和 G2/M 期，并触发细胞凋亡，这得到了 BAX 蛋白水平升高和 BCL-2 表达降低的支持。因此，GA-GNPs 在未来的 GBM 治疗研究中与放射治疗相结合具有很大的潜力。
