Daduang Jureerut, Palasap Adisak, Daduang Sakda, Boonsiri Patcharee, Suwannalert Prasit, Limpaiboon Temduang
Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand E-mail :
Asian Pac J Cancer Prev. 2015;16(1):169-74. doi: 10.7314/apjcp.2015.16.1.169.
Cervical cancer (CxCa) is the most common cancer in women and a prominent cause of cancer mortality worldwide. The primary cause of CxCa is human papillomavirus (HPV). Radiation therapy and chemotherapy have been used as standard treatments, but they have undesirable side effects for patients. It was reported that gallic acid has antioxidant, antimicrobial, and anticancer activities. Gold nanoparticles are currently being used in medicine as biosensors and drug delivery agents. This study aimed to develop a drug delivery agent using gold nanoparticles conjugated with gallic acid. The study was performed in uninfected (C33A) cervical cancer cells, cervical cancer cells infected with HPV type 16 (CaSki) or 18 (HeLa), and normal Vero kidney cells. The results showed that GA inhibited the proliferation of cancer cells by inducing apoptosis. To enhance the efficacy of this anticancer activity, 15-nm spherical gold nanoparticles (GNPs) were used to deliver GA to cancer cells. The GNPs-GA complex had a reduced ability compared to unmodified GA to inhibit the growth of CxCa cells. It was interesting that high-concentration (150 μM) GNPs-GA was not toxic to normal cells, whereas GA alone was cytotoxic. In conclusion, GNPs-GA could inhibit CxCa cell proliferation less efficiently than GA, but it was not cytotoxic to normal cells. Thus, gold nanoparticles have the potential to be used as phytochemical delivery agents for alternative cancer treatment to reduce the side effects of radiotherapy and chemotherapy.
宫颈癌（CxCa）是女性中最常见的癌症，也是全球癌症死亡的一个主要原因。宫颈癌的主要病因是人乳头瘤病毒（HPV）。放射治疗和化疗一直被用作标准治疗方法，但它们对患者有不良副作用。据报道，没食子酸具有抗氧化、抗菌和抗癌活性。金纳米颗粒目前在医学上用作生物传感器和药物递送剂。本研究旨在开发一种使用与没食子酸偶联的金纳米颗粒的药物递送剂。该研究在未感染的（C33A）宫颈癌细胞、感染16型（CaSki）或18型（HeLa）HPV的宫颈癌细胞以及正常的非洲绿猴肾细胞（Vero）中进行。结果表明，没食子酸通过诱导凋亡抑制癌细胞增殖。为了增强这种抗癌活性的功效，使用15纳米的球形金纳米颗粒（GNPs）将没食子酸递送至癌细胞。与未修饰的没食子酸相比，GNPs-没食子酸复合物抑制宫颈癌细胞生长的能力有所降低。有趣的是，高浓度（150μM）的GNPs-没食子酸对正常细胞无毒，而单独的没食子酸具有细胞毒性。总之，GNPs-没食子酸抑制宫颈癌细胞增殖的效率低于没食子酸，但对正常细胞无细胞毒性。因此，金纳米颗粒有潜力用作植物化学物质递送剂，用于替代癌症治疗，以减少放疗和化疗的副作用。
