Rattanata Narintorn, Daduang Sakda, Wongwattanakul Molin, Leelayuwat Chanvit, Limpaiboon Temduang, Lekphrom Ratsami, Sandee Alisa, Boonsiri Patcharee, Chio-Srichan Sirinart, Daduang Jureerut
Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand E-mail :
Asian Pac J Cancer Prev. 2015;16(16):7143-7. doi: 10.7314/apjcp.2015.16.16.7143.
Gold nanoparticles (GNPs) were conjugated with gallic acid (GA) at various concentrations between 30 and 150 μM and characterized using transmission electron microscopy (TEM) and UV-Vis spectroscopy (UV-VIS). The anticancer activities of the gallic acid-stabilized gold nanoparticles against well-differentiated (M213) and moderately differentiated (M214) adenocarcinomas were then determined using a neutral red assay. The GA mechanism of action was evaluated using Fourier transform infrared (FTIR) microspectroscopy. Distinctive features of the FTIR spectra between the control and GA-treated cells were confirmed by principal component analysis (PCA). The surface plasmon resonance spectra of the GNPs had a maximum absorption at 520 nm, whereas GNPs-GA shifted the maximum absorption values. In an in vitro study, the complexed GNPs-GA had an increased ability to inhibit the proliferation of cancer cells that was statistically significant (P<0.0001) in both M213 and M214 cells compared to GA alone, indicating that the anticancer activity of GA can be improved by conjugation with GNPs. Moreover, PCA revealed that exposure of the tested cells to GA resulted in significant changes in their cell membrane lipids and fatty acids, which may enhance the efficacy of this anticancer activity regarding apoptosis pathways.
将金纳米颗粒（GNPs）与浓度在30至150μM之间的没食子酸（GA）共轭，并使用透射电子显微镜（TEM）和紫外可见光谱（UV-VIS）进行表征。然后使用中性红测定法测定没食子酸稳定的金纳米颗粒对高分化（M213）和中分化（M214）腺癌的抗癌活性。使用傅里叶变换红外（FTIR）显微光谱法评估GA的作用机制。通过主成分分析（PCA）确认了对照细胞和GA处理细胞之间FTIR光谱的独特特征。GNPs的表面等离子体共振光谱在520nm处有最大吸收，而GNPs-GA使最大吸收值发生了偏移。在一项体外研究中，与单独的GA相比，复合的GNPs-GA在抑制癌细胞增殖方面的能力增强，在M213和M214细胞中均具有统计学意义（P<0.0001），这表明与GNPs共轭可以提高GA的抗癌活性。此外，PCA显示受试细胞暴露于GA会导致其细胞膜脂质和脂肪酸发生显著变化，这可能会增强这种抗癌活性在凋亡途径方面的功效。
