Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Universitat de València Valencia, Spain.
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Madrid, Spain.
Emerg Microbes Infect. 2021 Dec;10(1):407-415. doi: 10.1080/22221751.2020.1870411.
has arisen as an important multidrug-resistant fungus because of several nosocomial outbreaks and elevated rates of mortality. Accurate and rapid diagnosis of is highly desired; nevertheless, current methods often present severe limitations and produce misidentification. Herein a sensitive, selective, and time-competitive biosensor based on oligonucleotide-gated nanomaterials for effective detection of is presented. In the proposed design, a nanoporous anodic alumina scaffold is filled with the fluorescent indicator rhodamine B and the pores blocked with different oligonucleotides capable of specifically recognize genomic DNA. Gate opening modulation and cargo delivery is controlled by successful DNA recognition. is detected at a concentration as low as 6 CFU/mL allowing obtaining a diagnostic result in clinical samples in one hour with no prior DNA extraction or amplification steps.
已经成为一种重要的多药耐药真菌，因为它引起了几次医院感染爆发，并导致死亡率上升。人们非常希望能够准确、快速地诊断，然而，目前的方法往往存在严重的局限性，并导致错误识别。本文提出了一种基于寡核苷酸门控纳米材料的敏感、选择性和时间竞争型生物传感器，用于有效检测。在该设计中，纳米多孔阳极氧化铝支架中填充了荧光指示剂罗丹明 B，并通过不同的寡核苷酸将孔堵塞，这些寡核苷酸能够特异性识别 基因组 DNA。门控开启的调制和货物的输送由成功的 DNA 识别来控制。在 6 CFU/mL 的低浓度下即可检测到，允许在一个小时内从临床样本中获得诊断结果，无需事先进行 DNA 提取或扩增步骤。
