首次从叶子中分离得到具有显著抗类风湿关节炎作用的生物活性化合物。
Bioactive Compounds with Significant Anti- Rheumatoid Arthritis Effect Isolated for the First time from Leaves of Bougainvillea spectabilis.
机构信息
Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
Chemistry of Tanning Materials and Leather Technology, Department, National Research Centre, 33 El Bohouth st. (former EL Tahrir st.)-Dokki-Giza-Egypt-P.O.12622, Egypt.
出版信息
Curr Pharm Biotechnol. 2021;22(15):2048-2053. doi: 10.2174/1389201021666201229111825.
OBJECTIVE
The aim of this work is to identify and purify bioactive compounds from 70℅ aqueous methanolic extract (AME) of B. spectabilis leaves collected in Cairo, Egypt and assessed their rheumatoid arthritis activity.
METHODS
The methanolic extract of B. spectabilis leaves was fractionated and subjected to different chromatographic techniques to isolate pure new compounds which were identified by one dimensional and two dimensional nuclear magnetic resonance (NMR) spectroscopic analyses and mass spectrometric methods. The isolated compounds were evaluated for their anti-inflammatory activity on adjuvant induced chronic rheumatoid arthritis.
RESULTS & DISCUSSION: Seven bioactive compounds were purified and identified for the first time from the methanolic extract of Bougainvillea spectabilis leaves; secologanin dimethyl acetal, α- and β-amyrin, α- and β-amyrin acetate, Kaempferol and kaempferol-3-O-rhamnoside. The previously mentioned compounds had a significant effect against rheumatoid arthritis.
CONCLUSION
The seven compounds isolated from the methanolic extract of B. spectabilis leaves showed strong anti-rheumatoid arthritis activity.
目的
本工作旨在从埃及开罗采集的大红花叶片 70%甲醇提取物（AME）中鉴定和纯化具有生物活性的化合物，并评估其类风湿关节炎活性。
方法
对大红花叶片的甲醇提取物进行分段，并通过各种色谱技术进行分离，以分离出纯的新化合物，通过一维和二维核磁共振（NMR）光谱分析和质谱方法对其进行鉴定。对分离得到的化合物进行了评价，以评估其对佐剂诱导的慢性类风湿关节炎的抗炎活性。
结果与讨论
首次从大红花叶片的甲醇提取物中分离出 7 种具有生物活性的化合物；二甲缩醛_secologanin、α-和β-香树脂醇、α-和β-香树脂醇乙酸酯、山柰酚和山柰酚-3-O-鼠李糖苷。上述化合物对类风湿关节炎有显著的疗效。
结论
从大红花叶片甲醇提取物中分离得到的 7 种化合物具有很强的抗类风湿关节炎活性。
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