DST Sponsored Fish Nutrition Research Laboratory, Department of Zoology, University of Kashmir, Srinagar, India.
Department of Biochemistry, University of Kashmir, Srinagar, India.
J Fish Biol. 2021 May;98(5):1289-1302. doi: 10.1111/jfb.14661. Epub 2021 Jan 21.
Haematological and serum biochemical parameters are a valuable tool in determining the wellbeing status of different fish species and response in close association to various biological and environmental factors, therefore the aim of the present study was to establish sex-wise reference values of haemato-biochemical parameters of S. labiatus. The haematological parameters such as haemoglobin (Hb) concentration, total erythrocyte count (RBC), total leucocyte count (WBC), packed cell volume (PCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), mean corpuscular volume (MCV) and serum biochemical parameters such as glucose, cholesterol, urea, total protein, albumin and globulin were analysed. The results revealed that the haematological parameters, i.e., Hb, RBC, WBC and PCV showed significant (P < 0.05) differences with respect to sex. Significantly (P < 0.05) higher values of Hb content (11.18 g dl ), RBC count (1.88 × 10 mm ) and PCV (36.15%) were noted in male fish than females throughout the study period, while significantly (P < 0.05) higher WBC count was noted in females (20.38 × 10 mm ) compared to males. However, no significant (P > 0.05) difference was observed in erythrocyte indices like MCH, MCHC and MCV between sexes. The serum biochemical parameters, i.e., glucose, cholesterol and urea, also showed significant (P < 0.05) difference between the sexes, but no significant (P > 0.05) difference was noticed in total protein, albumin and globulin. Significantly (P < 0.05) highest values of glucose (116.76 mg dl ) and urea (9.01 mg dl ) were recorded in male S. labiatus, while highest value of cholesterol (223.53 mg dl ) was noted in females. The information generated in the present study gives the basic reference values of haematological and serum biochemical parameters of S. labiatus which will be useful in monitoring the wellbeing status of fish populations. Moreover, the data will also be helpful to ascertain the occurrence of different clinical and subclinical diseases, which may in turn help to boost the overall production of this species.
血液学和血清生化参数是确定不同鱼类物种健康状况和对各种生物和环境因素反应的有价值的工具,因此本研究的目的是确定 S. labiatus 的血液生化参数的性别参考值。血液学参数,如血红蛋白(Hb)浓度、总红细胞计数(RBC)、总白细胞计数(WBC)、红细胞压积(PCV)、平均红细胞血红蛋白(MCH)、平均红细胞血红蛋白浓度(MCHC)、平均红细胞体积(MCV)和血清生化参数,如葡萄糖、胆固醇、尿素、总蛋白、白蛋白和球蛋白进行了分析。结果表明,血液学参数,即 Hb、RBC、WBC 和 PCV 存在显著的性别差异(P<0.05)。整个研究期间,雄性鱼类的 Hb 含量(11.18g/dl)、RBC 计数(1.88×10 6 mm 3)和 PCV(36.15%)显著高于雌性(P<0.05),而 WBC 计数显著高于雌性(20.38×10 6 mm 3)比雄性(P<0.05)。然而,MCH、MCHC 和 MCV 等红细胞指数在性别之间没有观察到显著差异(P>0.05)。血清生化参数,如葡萄糖、胆固醇和尿素,在性别之间也存在显著差异(P<0.05),但总蛋白、白蛋白和球蛋白之间没有观察到显著差异(P>0.05)。雄性 S. labiatus 的葡萄糖(116.76mg/dl)和尿素(9.01mg/dl)含量最高,胆固醇(223.53mg/dl)含量最高的是雌性。本研究提供的信息为 S. labiatus 的血液学和血清生化参数提供了基本的参考值,这将有助于监测鱼类种群的健康状况。此外,这些数据还有助于确定不同临床和亚临床疾病的发生情况,从而有助于提高该物种的整体产量。