Fish Nutrition Research Laboratory, Department of Zoology, University of Kashmir, Hazratbal, Srinagar, 190 006 India.
Fish Nutrition Research Laboratory, Department of Zoology, University of Kashmir, Hazratbal, Srinagar, 190 006 India.
Tissue Cell. 2020 Dec;67:101398. doi: 10.1016/j.tice.2020.101398. Epub 2020 Jun 21.
The aim of the present study is to evaluate the effect of lithium heparin and KEDTA on haematology, biochemical parameters and blood cell morphology of snow trout Schizopyge plagiostomus inhabiting in Kashmir Himalayan region. The haematological parameters such as hemoglobin (Hb), red blood cellcount (RBC), white blood cell (WBC), haematocrit (Hct), erythrocyte indices; mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), produced certain significant differences in their values with respect to both anticoagulants. Higher values of Hct, MCH and MCV while lower RBC count and MCHC values were recorded with a sample treated with KEDTA compared to sample treated with lithium heparin. However, no significant change in Hb and WBC count was observed in the values of samples treated with both the anticoagulants. In the present study, the effect of two anticoagulant in biochemical parameters revealed that the alkaline phosphatase (ALP) and aspartate transaminase (AST) produced significant (P < 0.05) differences. Higher AST and lower ALP activity were recorded in the sample treated with KEDTA compared to overall values in serum. Morphometric analysis showed an increase in the RBC perimeter area in samples treated with KEDTA as compared to samples treated with lithium heparin. On the basis of above findings, it is recommended that in fish blood analysis heparin as anticoagulant could be useful as compared to K EDTA.
本研究旨在评估肝素锂和 KEDTA 对生活在克什米尔喜马拉雅地区的白斑狗鱼（Schizopyge plagiostomus）血液学、生化参数和血细胞形态的影响。血液学参数如血红蛋白（Hb）、红细胞计数（RBC）、白细胞（WBC）、血细胞比容（Hct）、红细胞指数；平均红细胞血红蛋白量（MCH）、平均红细胞血红蛋白浓度（MCHC）、平均红细胞体积（MCV），在两种抗凝剂的处理下，其值存在某些显著差异。与用肝素锂处理的样本相比，用 KEDTA 处理的样本中 Hct、MCH 和 MCV 值较高，而 RBC 计数和 MCHC 值较低。然而，用两种抗凝剂处理的样本中 Hb 和 WBC 计数的变化没有显著差异。在本研究中，两种抗凝剂对生化参数的影响表明，碱性磷酸酶（ALP）和天冬氨酸转氨酶（AST）产生了显著差异（P < 0.05）。与血清中的总体值相比，用 KEDTA 处理的样本中 AST 较高，而 ALP 活性较低。形态计量分析显示，与用肝素锂处理的样本相比，用 KEDTA 处理的样本中 RBC 周长面积增加。基于上述发现，建议在鱼类血液分析中，肝素作为抗凝剂比 K EDTA 更有用。
