Department of Nuclear Medicine Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, PR China.
Department of Cardiothoracic Surgery, The Affiliated Hospital of XuZhou Medical University, Xuzhou, PR China.
Colloids Surf B Biointerfaces. 2021 Mar;199:111542. doi: 10.1016/j.colsurfb.2020.111542. Epub 2020 Dec 26.
A novel form of magnetic bead, namely antibody-coated magnetic lipid nano-vehicle (AMLV), was synthesized by embedding FeO nanoparticles into an amphiphilic antibody-modified liposome as a high-performance circulating tumor cell (CTC) hunter. The CTC capture performance of AMLV was validated based on an enlarged patient sample (including 318 colorectal, 78 breast, 77 lung and 55 liver cancer patients) with high detection rate. The preliminary comparison with Cellsearch was also conducted, indicating that the cell membrane-semblance AMLV showed higher capture performance for different kinds of EpCAM-expressed circulating tumor cells in the peripheral blood (4.4 ± 1.2-fold for AMLV vs CellsearchTM, n=5, P<0.001). Moreover, the AMLV-isolated CTCs could be used as a functional material to provide various clinical information for tumor patients and work as an alternative of tumor tissue to conduct gene analysis after conventional PCR amplification.
一种新型的磁性珠，即抗体包被的磁性脂质纳米载体（AMLV），是通过将 FeO 纳米粒子嵌入到两亲性抗体修饰的脂质体中来合成的，作为高性能的循环肿瘤细胞（CTC）猎手。基于一个扩大的患者样本（包括 318 例结直肠癌、78 例乳腺癌、77 例肺癌和 55 例肝癌患者）进行了 AMLV 的 CTC 捕获性能验证，具有高检测率。还与 Cellsearch 进行了初步比较，表明细胞膜样 AMLV 对不同类型的 EpCAM 表达的外周血循环肿瘤细胞具有更高的捕获性能（AMLV 比 CellsearchTM 高 4.4 ± 1.2 倍，n=5，P<0.001）。此外，AMLV 分离的 CTC 可用作功能材料，为肿瘤患者提供各种临床信息，并在常规 PCR 扩增后替代肿瘤组织进行基因分析。
