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表皮生长因子受体靶向免疫磁性脂质体高效捕获循环结直肠肿瘤细胞。

Epidermal growth factor receptor-targeted immune magnetic liposomes capture circulating colorectal tumor cells efficiently.

机构信息

Department of Gastroenterology, Qilu Hospital of Shandong University, Qingdao 266035, Shandong Province, China.

Department of General Surgery, Qilu Hospital of Shandong University, Qingdao 266035, Shandong Province, China.

出版信息

World J Gastroenterol. 2018 Jan 21;24(3):351-359. doi: 10.3748/wjg.v24.i3.351.

DOI:10.3748/wjg.v24.i3.351
PMID:29391757
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5776396/
Abstract

AIM: To compare the capacity of newly developed epidermal growth factor receptor (EGFR)-targeted immune magnetic liposomes (EILs) epithelial cell adhesion molecule (EpCAM) immunomagnetic beads to capture colorectal circulating tumor cells (CTCs). METHODS: EILs were prepared using a two-step method, and the magnetic and surface characteristics were confirmed. The efficiency of capturing colorectal CTCs as well as the specificity were compared between EILs and EpCAM magnetic beads. RESULTS: The obtained EILs had a lipid nanoparticle structure similar to cell membrane. Improved binding with cancer cells was seen in EILs compared with the method of coupling nano/microspheres with antibody. The binding increased as the contact time extended. Compared with EpCAM immunomagnetic beads, EILs captured more CTCs in peripheral blood from colorectal cancer patients. The captured cells showed consistency with clinical diagnosis and pathology. Mutation analysis showed same results between captured CTCs and cancer tissues. CONCLUSION: EGFR antibody-coated magnetic liposomes show high efficiency and specificity in capturing colorectal CTCs.

摘要

目的：比较新型表皮生长因子受体（EGFR）靶向免疫磁脂（EIL）和上皮细胞黏附分子（EpCAM）免疫磁珠对捕获结直肠循环肿瘤细胞（CTC）的能力。

方法：采用两步法制备 EIL，并对其磁性和表面特性进行了确认。比较了 EIL 和 EpCAM 磁珠对结直肠 CTC 的捕获效率和特异性。

结果：得到的 EIL 具有类似细胞膜的脂质纳米颗粒结构。与纳米/微球与抗体偶联的方法相比，EIL 与癌细胞的结合能力增强。结合随接触时间的延长而增加。与 EpCAM 免疫磁珠相比，EIL 在结直肠癌患者外周血中捕获了更多的 CTC。捕获的细胞与临床诊断和病理学一致。突变分析显示，捕获的 CTC 和癌组织之间的结果相同。

结论：EGFR 抗体包被的磁性脂体在捕获结直肠 CTC 方面具有高效性和特异性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a46/5776396/13395fbabe8b/WJG-24-351-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a46/5776396/6c2a8d79a846/WJG-24-351-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a46/5776396/3871ae0ee7db/WJG-24-351-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a46/5776396/c25b1558bb60/WJG-24-351-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a46/5776396/ce7c873950ae/WJG-24-351-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a46/5776396/13395fbabe8b/WJG-24-351-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a46/5776396/6c2a8d79a846/WJG-24-351-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a46/5776396/3871ae0ee7db/WJG-24-351-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a46/5776396/c25b1558bb60/WJG-24-351-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a46/5776396/ce7c873950ae/WJG-24-351-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a46/5776396/13395fbabe8b/WJG-24-351-g005.jpg

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本文引用的文献

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Anal Chem. 2016-8-9

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