Department of Chemistry, American University of Beirut, Beirut, Lebanon.
Department of Chemistry, American University of Beirut, Beirut, Lebanon.
Colloids Surf B Biointerfaces. 2021 Mar;199:111546. doi: 10.1016/j.colsurfb.2020.111546. Epub 2020 Dec 21.
The developing public interest in traditional medicine, especially plants-based drug, has prompted extensive research on the potential of naturally existing compounds. Among these compounds, curcumin is currently one of the most studied substances. In this study, we elaborate the physical properties of diarachidonyl phosphatidyl choline (DAPC) liposome using fluorescence method, where curcumin at low concentration was used as a probe molecule. In the first place, the phase transition temperature of DAPC was determined by following the fluorescence intensity of curcumin as a function of temperature, along with evaluating the effect of concentration of curcumin in the presence or absence of chitosan oligosaccharide lactate as an additional protective layer. On the other hand, quenching reactions using CPB and KI as quenchers reflected the ease of entry of different concentrations of these quenchers to the curcumin located in the hydrophobic core of the liposome which give new insight about the lipophilicity and permeability of the DAPC membrane. Finally, the partition coefficient analysis was investigated. It was concluded that curcumin has a higher partition coefficient at a temperature above the phase transition temperature of DAPC liposomes where the liposome is in the fluid liquid crystalline phase. Modulation of liposomes properties in the presence of chitosan oligosaccharide lactate layer was for the first time investigated. Chitosan oligosaccharide lactate acts as protecting layer without changing the phase transition temperature, but it affects the membrane permeability depending on solid gel and liquid crystalline phase.
公众对传统医学（尤其是植物药）的兴趣日益浓厚，这促使人们对天然存在的化合物的潜力进行了广泛的研究。在这些化合物中，姜黄素是目前研究最多的物质之一。在这项研究中，我们使用荧光法详细阐述了二油酰基磷脂酰胆碱（DAPC）脂质体的物理性质，其中低浓度的姜黄素被用作探针分子。首先，通过随温度变化跟踪姜黄素的荧光强度来确定 DAPC 的相变温度，并评估在存在或不存在壳寡糖乳酸盐作为额外保护层的情况下，姜黄素浓度对其的影响。另一方面，使用 CPB 和 KI 作为猝灭剂的猝灭反应反映了不同浓度的猝灭剂进入位于脂质体疏水性核心的姜黄素的容易程度，这为 DAPC 膜的亲脂性和通透性提供了新的见解。最后，进行了分配系数分析。结果表明，在高于 DAPC 脂质体相变温度的温度下，姜黄素具有更高的分配系数，此时脂质体处于流动液晶相。首次研究了壳寡糖乳酸盐层存在下脂质体性质的调制。壳寡糖乳酸盐作为保护层，不会改变相变温度，但会根据固体凝胶和液晶相影响膜通透性。
