Frear Cody C, Zang Tuo, Griffin Bronwyn R, McPhail Steven M, Parker Tony J, Kimble Roy M, Cuttle Leila
Centre for Children's Burns and Trauma Research, South Brisbane, Queensland, Australia.
Faculty of Medicine, University of Queensland, Herston, Queensland, Australia.
Wound Repair Regen. 2021 Mar;29(2):288-297. doi: 10.1111/wrr.12887. Epub 2020 Dec 29.
Negative pressure wound therapy has been used to promote wound healing in a variety of settings, including as an adjunct to silver-impregnated dressings in the acute management of paediatric burns. Fluid aspirated by the negative pressure wound therapy system represents a potentially insightful research matrix for understanding the burn wound microenvironment and the intervention's biochemical mechanisms of action. The aim of this study was to characterize the proteome of wound fluid collected using negative pressure wound therapy from children with small-area thermal burns. Samples were obtained as part of a randomized controlled trial investigating the clinical efficacy of adjunctive negative pressure wound therapy. They were compared with blister fluid specimens from paediatric burn patients matched according to demographic and injury characteristics. Protein identification and quantification were performed via liquid chromatography tandem mass spectrometry and sequential window acquisition of all theoretical mass spectra data-independent acquisition. Proteins and biological pathways that were unique to or enriched in negative pressure wound therapy fluid samples were evaluated using principal components, partial least squares-discriminant, and gene ontology enrichment analyses. Eight viable samples of negative pressure wound therapy fluid were collected and analyzed with eight matched blister fluid samples. A total of 502 proteins were quantitatively profiled in the negative pressure wound therapy fluid, of which 444 (88.4%) were shared with blister fluid. Several proteins exhibited significant abundance differences between fluid types, with negative pressure wound therapy fluid showing a higher abundance of matrix metalloproteinase-9, arginase-1, low affinity immunoglobulin gamma Fc region receptor III-A, filamin-A, alpha-2-macroglobulin, and hemoglobin subunit alpha. The results lend support to the hypothesis that negative pressure wound therapy augments wound healing through the modulation of factors involved in the inflammatory response, granulation tissue synthesis, and extracellular matrix maintenance. Data are available via ProteomeXchange with identifier PXD023168.
负压伤口治疗已被用于在各种情况下促进伤口愈合，包括在小儿烧伤的急性处理中作为含银敷料的辅助手段。负压伤口治疗系统吸出的液体是一种潜在的有洞察力的研究基质，有助于了解烧伤伤口微环境和该干预措施的生化作用机制。本研究的目的是对使用负压伤口治疗从小面积热烧伤儿童中收集的伤口液体蛋白质组进行表征。样本是作为一项调查负压伤口治疗辅助临床疗效的随机对照试验的一部分获得的。将它们与根据人口统计学和损伤特征匹配的小儿烧伤患者的水疱液标本进行比较。通过液相色谱串联质谱和所有理论质谱数据独立采集的顺序窗口获取进行蛋白质鉴定和定量。使用主成分分析、偏最小二乘判别分析和基因本体富集分析对负压伤口治疗液体样本中独特或富集的蛋白质和生物途径进行评估。收集了8份负压伤口治疗液体的有效样本，并与8份匹配的水疱液样本进行分析。在负压伤口治疗液体中总共对502种蛋白质进行了定量分析，其中444种（88.4%）与水疱液共有。几种蛋白质在不同类型的液体中表现出显著的丰度差异，负压伤口治疗液体中基质金属蛋白酶-9、精氨酸酶-1、低亲和力免疫球蛋白γFc区受体III-A、细丝蛋白-A、α-2-巨球蛋白和血红蛋白亚基α的丰度较高。这些结果支持了这样的假设，即负压伤口治疗通过调节参与炎症反应、肉芽组织合成和细胞外基质维持的因子来促进伤口愈合。数据可通过ProteomeXchange获得，标识符为PXD023168。
