Jia Tong, Yao Yu-Shan, Wang Rui-Hong
Shanxi Key Laboratory of Ecological Restoration on Loess Plateau, Institute of Loess Plateau, Shanxi University, Taiyuan 030006, China.
Huan Jing Ke Xue. 2020 Dec 8;41(12):5628-5635. doi: 10.13227/j.hjkx.202005006.
The phyllosphere and rhizosphere of plants and their living environment jointly form a complex ecosystem. Rhizosphere microorganisms are also the main driving force of the circulation of soil materials, which can provide a basis for the growth and development of plants. Phyllosphere and rhizosphere microorganisms can also be used as ecological indicators, and play significant roles in the ecological stability and recovery of mining areas. In this study, we selected a dominant species, , as the research object. We studied the characteristics of phyllosphere and rhizosphere bacterial communities in from copper tailings with high-throughput sequencing methods. We explored the key ecological factors affecting the structure and diversity of phyllosphere and rhizosphere bacterial communities in . The results showed that there were significant differences in the bacterial community structures between the rhizosphere and phyllosphere. The dominant phyllosphere bacterial genera of included , , and . The dominant rhizosphere bacterial genera were and . Moreover, the Shannon diversity, abundance-based coverage estimator (ACE), and Chao1 indices of rhizosphere bacterial communities were significantly higher than those of phyllosphere communities. The key ecological factors affecting the dominant phyllosphere and rhizosphere bacterial genera included soil water content, pH, soil arsenic and zinc, total nitrogen, and sulfur of , as well as plant cadmium and chromium. Furthermore, the Shannon diversity indices of rhizosphere bacterial communities were negatively correlated with root copper contents, and Simpson indices were positively correlated with root total nitrogen. There was a significant positive correlation between the ACE index and leaf total sculpture. These results provide a scientific basis for the exploration and utilization of phyllosphere and rhizosphere bacterial resources, and could improve the efficiency of ecological restoration in copper tailings.
植物的叶际和根际及其生存环境共同构成一个复杂的生态系统。根际微生物也是土壤物质循环的主要驱动力，可为植物的生长发育提供基础。叶际和根际微生物还可作为生态指标，在矿区生态稳定和恢复中发挥重要作用。在本研究中，我们选择了一个优势物种 作为研究对象。我们采用高通量测序方法研究了 铜尾矿中叶际和根际细菌群落的特征。我们探讨了影响 中叶际和根际细菌群落结构和多样性的关键生态因素。结果表明，根际和叶际细菌群落结构存在显著差异。 的优势叶际细菌属包括 、 和 。优势根际细菌属为 和 。此外，根际细菌群落的香农多样性、基于丰度的覆盖率估计器（ACE）和Chao1指数显著高于叶际群落。影响优势叶际和根际细菌属的关键生态因素包括土壤含水量、pH值、土壤砷和锌、 中的总氮和硫，以及植物镉和铬。此外，根际细菌群落的香农多样性指数与根铜含量呈负相关，辛普森指数与根总氮呈正相关。ACE指数与叶总雕塑之间存在显著正相关。这些结果为叶际和根际细菌资源的开发利用提供了科学依据，有助于提高铜尾矿生态修复效率。
