Obesity-Mediated Disease Research (ODR) Center, School of Medicine, Keimyung University, Daegu 42601, Korea.
Department of Biomedical Engineering, School of Medicine, Keimyung University, Daegu 42601, Korea.
Biosensors (Basel). 2020 Dec 24;11(1):7. doi: 10.3390/bios11010007.
Leptin is a peptide hormone produced primarily in adipose tissues. Leptin is considered a biomarker associated with obesity and obesity-mediated diseases. Biosensor detection of leptin in the blood may play a critical role as an indicator of dynamic pathological changes. In this paper, we introduce an electrochemical biosensor that adopts o-Phenylenediamine (oPD) on screen-printed gold electrodes (SPGEs) for detecting the leptin from a mouse model of diet-induced obesity (DIO). A linear calibration curve for the leptin concentration was obtained in the ranges from 0.1 to 20 ng/mL with a lower detection limit of 0.033 ng/mL. The leptin concentration was quantified with HRP (horseradish peroxidase)-catalyzed oxidation of oPD by two voltammetry methods: cyclic voltammetry (CV) and square-wave voltammetry (SWV). The proposed sandwich enzyme-linked immunosorbent assay (ELISA)-based electrochemical biosensor for the leptin in mouse blood serum showed high stability, sensitivity, selectivity, and effectivity compared to the commercial Leptin ELISA measurement. Thus, we believe that this leptin biosensor can be a sensitive analytical tool to detect low-levels of biomarkers in clinics and point-of-care testing (POCT).
瘦素是一种主要在脂肪组织中产生的肽激素。瘦素被认为是与肥胖和肥胖介导的疾病相关的生物标志物。血液中瘦素的生物传感器检测可能作为动态病理变化的指标发挥关键作用。在本文中，我们介绍了一种电化学生物传感器，该传感器采用邻苯二胺（oPD）在丝网印刷金电极（SPGE）上用于检测饮食诱导肥胖（DIO）小鼠模型中的瘦素。在 0.1 至 20 ng/mL 的范围内获得了瘦素浓度的线性校准曲线，检测下限为 0.033 ng/mL。通过两种伏安法：循环伏安法（CV）和方波伏安法（SWV），用辣根过氧化物酶（HRP）催化 oPD 的氧化来定量瘦素。与商业瘦素 ELISA 测量相比，基于夹心酶联免疫吸附测定（ELISA）的用于检测小鼠血清中瘦素的电化学生物传感器具有更高的稳定性、灵敏度、选择性和有效性。因此，我们相信这种瘦素生物传感器可以成为一种灵敏的分析工具，用于检测临床和即时检测（POCT）中的低水平生物标志物。
