Pratiwi Feby Wijaya, Peng Chien-Chung, Wu Si-Han, Kuo Chiung Wen, Mou Chung-Yuan, Tung Yi-Chung, Chen Peilin
Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan.
Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 11031, Taiwan.
Biomedicines. 2020 Dec 24;9(1):10. doi: 10.3390/biomedicines9010010.
Mesoporous silica nanoparticles (MSNs) have emerged as a prominent nanomedicine platform, especially for tumor-related nanocarrier systems. However, there is increasing concern about the ability of nanoparticles (NPs) to penetrate solid tumors, resulting in compromised antitumor efficacy. Because the physicochemical properties of NPs play a significant role in their penetration and accumulation in solid tumors, it is essential to systematically study their relationship in a model system. Here, we report a multihierarchical assessment of the accumulation and penetration of fluorescence-labeled MSNs with nine different physicochemical properties in tumor spheroids using two-photon microscopy. Our results indicated that individual physicochemical parameters separately could not define the MSNs' ability to accumulate in a deeper tumor region; their features are entangled. We observed that the MSNs' stability determined their success in reaching the hypoxia region. Moreover, the change in the MSNs' penetration behavior postprotein crowning was associated with both the original properties of NPs and proteins on their surfaces.
介孔二氧化硅纳米颗粒（MSNs）已成为一个重要的纳米医学平台，特别是用于肿瘤相关的纳米载体系统。然而，人们越来越关注纳米颗粒（NPs）穿透实体瘤的能力，这导致抗肿瘤疗效受损。由于NPs的物理化学性质在其在实体瘤中的渗透和积累中起着重要作用，因此在模型系统中系统地研究它们之间的关系至关重要。在此，我们报告了使用双光子显微镜对具有九种不同物理化学性质的荧光标记MSNs在肿瘤球体中的积累和渗透进行的多层次评估。我们的结果表明，单个物理化学参数不能单独定义MSNs在更深肿瘤区域积累的能力；它们的特征相互纠缠。我们观察到，MSNs的稳定性决定了它们到达缺氧区域的成功率。此外，蛋白质加帽后MSNs渗透行为的变化与NPs的原始性质及其表面的蛋白质都有关。
