Department of Chemistry, Kyungpook National University, Daegu 41566, Korea.
Department of Environment, Munshiganj District Office, Munshiganj-1500, Bangladesh.
Int J Mol Sci. 2020 Dec 24;22(1):132. doi: 10.3390/ijms22010132.
High-resolution mass spectrometry equipped with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources was used to enhance the characterization of phytochemicals of ethanol extracts of L. leaves (ZLE). Sugar compounds, dicarboxylic acids, compounds of phenolic acids and flavonoids groups, and other phytochemicals were detected from the leaves. Antioxidant activity and inhibition potentiality of ZLE against α-glucosidase enzyme, and elastase enzyme activities were evaluated in in vitro analysis. ZLE significantly inhibited activities of α-glucosidase enzyme at a lower concentration (IC 2.51 ± 0.15 µg/mL). Glucose uptake in C2C12 cells was significantly enhanced by 42.13 ± 0.15% following the treatment with ZLE at 30 µg/mL. It also exhibited potential antioxidant activities and elastase enzyme inhibition activity (IC 27.51 ± 1.70 µg/mL). Atmospheric pressure chemical ionization mass spectrometry (APCI-MS) detected more peaks than electrospray ionization mass spectrometry (ESI-MS), and both ionization techniques illustrated the biological activities of the detected compounds more thoroughly compared to single-mode analysis. Our findings suggest that APCI along with ESI is a potential ionization technique for metabolite profiling, and ZLE has the potential in managing diabetes by inhibiting α-glucosidase activity and enhancing glucose uptake.
采用配有电喷雾电离（ESI）和大气压化学电离（APCI）源的高分辨率质谱法来增强对 L. 叶（ZLE）乙醇提取物中植物化学成分的表征。从叶片中检测到糖化合物、二羧酸、酚酸和黄酮类化合物的化合物以及其他植物化学成分。在体外分析中，评估了 ZLE 的抗氧化活性和对α-葡萄糖苷酶的抑制潜力以及弹性蛋白酶酶活性。ZLE 在较低浓度（IC 2.51 ± 0.15 µg/mL）下显著抑制α-葡萄糖苷酶的活性。用 30 µg/mL 的 ZLE 处理后，C2C12 细胞中的葡萄糖摄取显著增强了 42.13 ± 0.15%。它还表现出潜在的抗氧化活性和弹性蛋白酶抑制活性（IC 27.51 ± 1.70 µg/mL）。大气压化学电离质谱（APCI-MS）比电喷雾电离质谱（ESI-MS）检测到更多的峰，与单模式分析相比，这两种电离技术更全面地说明了所检测化合物的生物活性。我们的研究结果表明，APCI 与 ESI 一起是代谢物分析的潜在电离技术，ZLE 通过抑制α-葡萄糖苷酶活性和增强葡萄糖摄取来具有治疗糖尿病的潜力。
