Miranda-Nieves David, Chaikof Elliot L
Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
Department of Surgery, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, United States.
ACS Biomater Sci Eng. 2017 May 8;3(5):694-711. doi: 10.1021/acsbiomaterials.6b00250. Epub 2016 Jul 27.
Collagen and elastin represent the two most predominant proteins in the body and are responsible for modulating important biological and mechanical properties. Thus, the focus of this review is the use of collagen and elastin as biomaterials for the fabrication of living tissues. Considering the importance of both biomaterials, we first propose the notion that many tissues in the human body represent a reinforced composite of collagen and elastin. In the rest of the review, collagen and elastin biosynthesis and biophysics, as well as molecular sources and biomaterial fabrication methodologies, including casting, fiber spinning, and bioprinting, are discussed. Finally, we summarize the current attempts to fabricate a subset of living tissues and, based on biochemical and biomechanical considerations, suggest that future tissue-engineering efforts consider direct incorporation of collagen and elastin biomaterials.
胶原蛋白和弹性蛋白是人体中两种最主要的蛋白质，负责调节重要的生物学和力学特性。因此，本综述的重点是将胶原蛋白和弹性蛋白用作制造活组织的生物材料。考虑到这两种生物材料的重要性，我们首先提出这样一种观点，即人体中的许多组织都是胶原蛋白和弹性蛋白的增强复合材料。在综述的其余部分，将讨论胶原蛋白和弹性蛋白的生物合成与生物物理学，以及分子来源和生物材料制造方法，包括铸造、纤维纺丝和生物打印。最后，我们总结了目前制造一部分活组织的尝试，并基于生化和生物力学方面的考虑，建议未来的组织工程工作考虑直接加入胶原蛋白和弹性蛋白生物材料。
