Department of Horticulture, Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University, Jinju 52828, Korea.
Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea.
Int J Mol Sci. 2020 Dec 24;22(1):133. doi: 10.3390/ijms22010133.
is a valuable hardwood plant with a high economical value for its medicinal and ornamental qualities. The aim of this work was to investigate the effects of the iron (Fe) source and medium pH on the growth and development of in vitro. The Fe sources used, including non-chelated iron sulfate (FeSO), iron ethylenediaminetetraacetic acid (Fe-EDTA), and iron diethylenetriaminepentaacetic acid (Fe-DTPA), were supplemented to the Multipurpose medium with a final Fe concentration of 2.78 mg·L. The medium without any supplementary Fe was used as the control. The pH of the agar-solidified medium was adjusted to either 4.70, 5.70, or 6.70. The experiment was conducted in a culture room for six weeks with 25 °C day and night temperatures, and a 16-h photoperiod with a light intensity of 50 mmol·m·s photosynthetic photon flux density (PPFD). Both the Fe source and pH affected the growth and development of the micropropagated plants in vitro. The leaves were greener in the pH 4.70 and 5.70 treatments. The tissue Fe content decreased with the increase of the medium pH. The leaf chlorophyll content was similar between plants treated with FeSO and those with Fe-EDTA. The numbers of the shoots and roots of plantlets treated with FeSO were 2.5 and 2 times greater than those of the control, respectively. The fresh and dry weights of the shoot and the root were the greatest for plants treated with Fe-EDTA combined with pH 5.70. The calcium, magnesium, and manganese contents in the plantlets increased in the pH 5.70 treatments regardless of the Fe source. Supplementary Fe decreased the activity of ferric chelate reductase. Overall, although the plantlets absorbed more Fe at pH 4.70, Fe-EDTA combined with pH 5.70 was found to be the best for the growth and development of in vitro.
是一种具有高经济价值的珍贵硬木植物，因其药用和观赏品质而受到重视。本工作旨在研究铁（Fe）源和培养基 pH 值对 体外生长发育的影响。使用的铁源包括非螯合硫酸亚铁（FeSO）、铁乙二胺四乙酸（Fe-EDTA）和铁二乙三胺五乙酸（Fe-DTPA），添加到终浓度为 2.78mg·L 的通用培养基中。不添加任何补充铁的培养基用作对照。琼脂固化培养基的 pH 值分别调整为 4.70、5.70 或 6.70。实验在培养室中进行了六周，白天和夜间温度为 25°C，光周期为 16 小时，光强为 50mmol·m·s 光合光子通量密度（PPFD）。铁源和 pH 值都影响了体外微繁殖植物的生长和发育。pH 值为 4.70 和 5.70 的处理中叶片更绿。组织铁含量随培养基 pH 值的升高而降低。用 FeSO 和 Fe-EDTA 处理的植株叶片叶绿素含量相似。用 FeSO 处理的植株的新芽和根的数量分别比对照多 2.5 倍和 2 倍。用 Fe-EDTA 处理并结合 pH 值为 5.70 的植株的地上部和根的鲜重和干重最大。无论铁源如何，pH 值为 5.70 的处理都能增加植株钙、镁和锰的含量。总的来说，尽管在 pH 值为 4.70 时植株吸收了更多的铁，但发现 Fe-EDTA 结合 pH 值 5.70 最有利于 体外生长和发育。
