Department of Plant Physiology, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
Plant Physiol. 1985 Jul;78(3):596-600. doi: 10.1104/pp.78.3.596.
A rapid and simple method for the determination of a ferric iron pool in the free space of roots is described. Formation of this pool depended on the source of iron in the nutrient solution. During growth in water culture at pH 5 to 6 with Fe-ethylenediaminetetraacetate, a free space pool of 500 to 1000 nanomoles Fe per gram fresh weight was formed in the roots of bean (Phaseolus vulgaris L. var. Prélude), maize (Zea mays L. var. Capella), and chlorophytum (Chlorophytum comosum [Thunb.] Jacques). No significant pool (less than 100 nanomoles per gram fresh weight) was formed with ferrioxamine. Upon impending Fe deficiency, bean and chlorophytum were able to mobilize this pool. Fe-deficient bean plants mobilized iron from the free space iron pool of another plant in the same vessel.
描述了一种快速而简单的方法来测定根系自由空间中的铁池。该铁池的形成取决于营养液中铁的来源。在 pH 值为 5 至 6 的水培条件下，用乙二胺四乙酸铁生长时，菜豆（Phaseolus vulgaris L. var. Prélude）、玉米（Zea mays L. var. Capella）和吊兰（Chlorophytum comosum [Thunb.] Jacques）的根中形成了 500 至 1000 纳摩尔每克鲜重的自由空间铁池。用高铁胺则不会形成明显的铁池（每克鲜重少于 100 纳摩尔）。在即将发生缺铁时，菜豆和吊兰能够动员这个铁池。缺铁的菜豆植物能够从同一容器中另一种植物的自由空间铁池中动员铁。
