用于基于酶联和磁性颗粒的免疫测定的微流控平台

Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay.

作者信息

Bhalla Nikhil, Chung Danny Wen Yaw, Chang Yaw-Jen, Uy Kimberly Jane S, Ye Yi Ying, Chin Ting-Yu, Yang Hao Chun, Pijanowska Dorota G

机构信息

Department of Electronic Engineering, Chung Yuan Christian University, Zhongli 32023, Taiwan.

Department of Mechanical Engineering, Chung Yuan Christian University, Zhongli 32023, Taiwan.

出版信息

Micromachines (Basel). 2013 Jun 18;4(2):257-271. doi: 10.3390/mi4020257.

DOI:10.3390/mi4020257

PMID:33374491

Abstract

This article presents design and testing of a microfluidic platform for immunoassay. The method is based on sandwiched ELISA, whereby the primary antibody is immobilized on nitrocelluose and, subsequently, magnetic beads are used as a label to detect the analyte. The chip takes approximately 2 h and 15 min to complete the assay. A Hall Effect sensor using 0.35-μm BioMEMS TSMC technology (Taiwan Semiconductor Manufacturing Company Bio-Micro-Electro-Mechanical Systems) was fabricated to sense the magnetic field from the beads. Furthermore, florescence detection and absorbance measurements from the chip demonstrate successful immunoassay on the chip. In addition, investigation also covers the Hall Effect simulations, mechanical modeling of the bead-protein complex, testing of the microfluidic platform with magnetic beads averaging 10 nm, and measurements with an inductor-based system.

摘要

本文介绍了一种用于免疫测定的微流控平台的设计与测试。该方法基于夹心酶联免疫吸附测定法，即首先将一抗固定在硝酸纤维素上，随后使用磁珠作为标记物来检测分析物。该芯片完成测定大约需要2小时15分钟。采用0.35微米台积电生物微机电系统技术制造了一个霍尔效应传感器，用于感应磁珠产生的磁场。此外，芯片的荧光检测和吸光度测量结果表明在芯片上成功实现了免疫测定。此外，研究还包括霍尔效应模拟、磁珠 - 蛋白质复合物的力学建模、对平均粒径为10纳米的磁珠的微流控平台测试以及基于电感系统的测量。