Pack Chan-Gi, Paulson Bjorn, Shin Yeonhee, Jung Min Kyo, Kim Jun Sung, Kim Jun Ki
Asan Institute for Life Science, Asan Medical Center, Seoul 05505, Korea.
Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul 05505, Korea.
Materials (Basel). 2020 Dec 23;14(1):19. doi: 10.3390/ma14010019.
Controlling the uptake of nanoparticles into cells so as to balance therapeutic effects with toxicity is an essential unsolved problem in the development of nanomedicine technologies. From this point of view, it is useful to use standard nanoparticles to quantitatively evaluate the physical properties of the nanoparticles in solution and in cells, and to analyze the intracellular dynamic motion and distribution of these nanoparticles at a single-particle level. In this study, standard nanoparticles are developed based on a variant silica-based nanoparticle incorporating fluorescein isothiocyanate (FITC) or/and rhodamine B isothiocyanate (RITC) with a variety of accessible diameters and a matching fluorescent cobalt ferrite core-shell structure (FeO/SiO). The physical and optical properties of the nanoparticles in vitro are fully evaluated with the complementary methods of dynamic light scattering, electron microscopy, and two fluorescence correlation methods. In addition, cell uptake of dual-colored and core/shell nanoparticles via endocytosis in live HeLa cells is detected by fluorescence correlation spectroscopy and electron microscopy, indicating the suitability of the nanoparticles as standards for further studies of intracellular dynamics with multi-modal methods.
在纳米医学技术的发展中，控制纳米颗粒进入细胞的过程，从而平衡治疗效果与毒性，是一个尚未解决的关键问题。从这一角度来看，使用标准纳米颗粒来定量评估溶液中和细胞内纳米颗粒的物理性质，并在单颗粒水平上分析这些纳米颗粒在细胞内的动态运动和分布是很有意义的。在本研究中，基于一种变体二氧化硅基纳米颗粒开发了标准纳米颗粒，该颗粒结合了异硫氰酸荧光素（FITC）或/和异硫氰酸罗丹明B（RITC），具有多种可及直径，并具有匹配的荧光钴铁氧体核壳结构（FeO/SiO）。通过动态光散射、电子显微镜和两种荧光相关方法等互补手段，对纳米颗粒在体外的物理和光学性质进行了全面评估。此外，通过荧光相关光谱和电子显微镜检测了双色核壳纳米颗粒在活HeLa细胞中通过内吞作用的细胞摄取情况，表明这些纳米颗粒适合作为进一步使用多模态方法研究细胞内动力学的标准。
